Transcriptomics,Genomics

Dataset Information

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Expression data from MCF-7 breast cancer cells treated with Wild Type Tomato fruit extract (WT) and transgenic lines tomato fruit extract (L6 and L7)


ABSTRACT: To investigate the effects of transgenic lines L6 and L7 tomato fruits on total expression profile of MCF-7 breast cancer cells, we treated MCF-7 cells with 1 ug/ml of tomato fruit extract for 24 hours and compare it with wild type tomato fruit extract Objectives for this study included the identification of genes that were up or down-regulated at the transcriptional level in MCF-7 cells treated with transgenic lines L6 and L7 tomatofruit extract and compare it to wild type tomato fruit extract. Overall design: MCF-7 breast cancer cells were incubated with wild type (WT) tomatofruit extract (1 ug/ml), line 6 (L6) transgenic line tomato fruit extract (1 ug/ml) and line 7 (L7) transgenic line tomato fruit extract (1 ug/ml) for 24 hours and selected for RNA extraction and hybridization on Affymetrix microarrays. cell death responses

INSTRUMENT(S): [PrimeView] Affymetrix Human Gene Expression Array

SUBMITTER: Mariya Khodakovskaya  

PROVIDER: GSE94548 | GEO | 2017-06-12

SECONDARY ACCESSION(S): PRJNA371453

REPOSITORIES: GEO

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Publications

Polyphenolic extract of InsP 5-ptase expressing tomato plants reduce the proliferation of MCF-7 breast cancer cells.

Alimohammadi Mohammad M   Lahiani Mohamed Hassen MH   McGehee Diamond D   Khodakovskaya Mariya M  

PloS one 20170427 4


In recent years, by extensive achievements in understanding the mechanisms and the pathways affected by cancer, the focus of cancer research is shifting from developing new chemotherapy methods to using natural compounds with therapeutic properties to reduce the adverse effects of synthetic drugs on human health. We used fruit extracts from previously generated human type I InsP 5-ptase gene expressing transgenic tomato plants for assessment of the anti-cancer activity of established genetically  ...[more]

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