Genomics

Dataset Information

0

RNA-seq profiling of rexinoid responsive gene expression during early myogenic differentiation


ABSTRACT: While skeletal myogenesis is tightly coordinated by myogenic regulatory factors including MyoD and myogenin, chromatin modifications have emerged as vital mechanisms of myogenic regulation. We have previously established that bexarotene, a clinically approved agonist of retinoid X receptor, promotes the specification and differentiation of skeletal muscle lineage. Here, we examine a genome-wide impact of rexinoids on myogenic differentiation through integral RNA-seq and ChIP-seq analyses. We found that bexarotene promotes myoblast differentiation through the coordination of exit from the cell cycle and the activation of muscle-related genes. We uncovered a new mechanism of rexinoid action which is mediated by the nuclear receptor and largely reconciled through a direct regulation of MyoD gene expression. In addition, we determined a rexinoid-responsive residue-specific histone acetylation at a distinct chromatin state associated to MyoD and myogenin. Thus, we provide novel molecular insights into the interplay between retinoid X receptor signaling and chromatin states pertinent to myogenic programs in early myoblast differentiation.

ORGANISM(S): Mus musculus

PROVIDER: GSE94560 | GEO | 2017/09/13

SECONDARY ACCESSION(S): PRJNA371489

REPOSITORIES: GEO

Similar Datasets

2017-09-13 | GSE94558 | GEO
2020-06-24 | GSE139942 | GEO
2017-04-28 | GSE94779 | GEO
2018-06-01 | GSE113248 | GEO
| E-GEOD-50413 | biostudies-arrayexpress
| E-GEOD-10430 | biostudies-arrayexpress
2006-03-01 | GSE3858 | GEO
| PRJNA371483 | ENA
2020-07-14 | PXD006711 | Pride
2008-03-01 | GSE10430 | GEO