Genomics

Dataset Information

34

A non-catalytic role of TET3 promotes open chromatin and enhances global transcription


ABSTRACT: The methylcytosine dioxygenase Tet3 is highly expressed as a specific isoform in oocytes and zygotes but essentially absent from later stages of mouse preimplantation development. Here, we show that Tet3 expression promotes transdifferentiation of embryonic stem cells to trophoblast-like stem cells. By genome-wide analyses we demonstrate that TET3 associates with and co-occupies chromatin with RNA Polymerase II. Tet3 expression induces a global increase of transcription and total RNA levels, and its presence further enhances chromatin accessibility in regions open for transcription. This novel function of TET3 is not specific to the oocyte isoform, independent of its catalytic activity, the CXXC domain, or its interaction with OGT, and is localised in its highly conserved exon 4. We propose a more general role for TET3 promoting open chromatin and enhancing global transcription during changes of cellular identity, separate from its catalytic function. Overall design: Transcriptome, ChIP and chromatin accessibility profiling for ESC overexpressing TET3

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

SUBMITTER: Christel Krueger  

PROVIDER: GSE94688 | GEO | 2018-05-31

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
GSE94688_RPM_log2_500bp_tiles_ATAC.txt.gz Txt
GSE94688_RPM_log2_500bp_tiles_ChIP.txt.gz Txt
GSE94688_RPM_log2_RNA_seq.txt.gz Txt
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