Transcriptomics,Genomics

Dataset Information

44

Global transcriptome profiling identifies KLF15 and SLC25A10 as regulators of adipocytes insulin sensitivity in obese women [WAT]


ABSTRACT: The aim of this study was to identify new genes controlling insulin sensitivity in adipocytes from obese women with either insulin-resistant (OIR) or -sensitive (OIS) adipocytes. 432 genes were differentially expressed between the OIR and OIS group (FDR <5%). These genes are enriched in pathways related to glucose and amino acid metabolism, cellular respiration, and insulin signaling, and include SLC2A4, AKT2, as well as enzymes in the mitochondria respiratory chain. Two IR-associated genes, KLF15 and SLC25A10, were selected for functional evaluation. Overall design: Insulin sensitivity was determined by measuring lipogenesis in isolated adipocytes from abdominal subcutaneous white adipose tissue (WAT). We performed microarray-based transcriptome profiling on subcutaneous WAT specimen from 9 lean, 21 OIS and 18 obese OIR women. Patients were examined in the morning after 12 hrs fasting. An abdominal subcutaneous WAT biopsy was obtained by needle aspiration. From WAT specimen we prepared and hybridised biotinylated complementary RNA to GeneChip Human Gene 1.1 ST Arrays (Affymetrix Inc., Santa Clara, CA), and then washed, stained and scanned the slides using standardised protocols (Affymetrix Inc.). Significance analysis of microarrays (SAM) was used to compare the difference in gene expression between groups.

INSTRUMENT(S): [HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version]

SUBMITTER: Ingrid Dahlman  

PROVIDER: GSE94752 | GEO | 2017-06-29

SECONDARY ACCESSION(S): PRJNA373929

REPOSITORIES: GEO

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Publications

Global transcriptome profiling identifies KLF15 and SLC25A10 as modifiers of adipocytes insulin sensitivity in obese women.

Kulyté Agné A   Ehrlund Anna A   Arner Peter P   Dahlman Ingrid I  

PloS one 20170601 6


Although the mechanisms linking obesity to insulin resistance (IR) and type 2 diabetes (T2D) are not entirely understood, it is likely that alterations of adipose tissue function are involved. The aim of this study was to identify new genes controlling insulin sensitivity in adipocytes from obese women with either insulin resistant (OIR) or sensitive (OIS) adipocytes. Insulin sensitivity was first determined by measuring lipogenesis in isolated adipocytes from abdominal subcutaneous white adipos  ...[more]

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