Transcriptomics

Dataset Information

0

A Reciprocal Regulatory Loop Between TAZ/YAP and G protein Gαs Regulates Schwann Cell Proliferation and Differentiation


ABSTRACT: Schwann cell (SC) myelination in the peripheral nervous system is essential for motor function, and uncontrolled SC proliferation occurs in cancer. Here, we show that a dual direct role for Hippo effectors TAZ and YAP in regulation of SC proliferation and myelination through modulating G protein expression and interacting with SOX10, respectively. Developmentally-regulated mutagenesis indicates that TAZ/YAP are critical for SC proliferation yet also required for their differentiation and myelination. Genome-wide occupancy mapping and transcriptome profiling reveal that nuclear TAZ and YAP promote SC proliferation by activating cell cycle regulators, while targeting critical differentiation regulators in cooperation with SOX10 for myelination. We further identified that TAZ targets and represses Gnas, encoding Gαs-protein, which opposes TAZ/YAP activities to decelerate proliferation. Gnas deletion expands SC precursor pools and blocks myelination in the sciatic nerve. Thus, our study revealed that the Hippo/TAZ/YAP and Gαs-protein feedback circuit functions as a fulcrum balancing SC proliferation and differentiation, providing insights into molecular programming of SC lineage progression and homeostasis.

ORGANISM(S): Mus musculus Rattus norvegicus

PROVIDER: GSE94990 | GEO | 2017/04/26

SECONDARY ACCESSION(S): PRJNA375093

REPOSITORIES: GEO

Similar Datasets

2016-06-06 | GSE79115 | GEO
2017-06-20 | E-MTAB-5395 | biostudies-arrayexpress
2018-02-13 | GSE99040 | GEO
2017-06-13 | PXD005751 | Pride
2014-07-18 | E-GEOD-45155 | biostudies-arrayexpress
2013-12-31 | E-GEOD-49384 | biostudies-arrayexpress
2019-06-17 | E-MTAB-7614 | biostudies-arrayexpress
2021-10-23 | GSE166736 | GEO
2022-02-12 | GSE196262 | GEO
2023-10-04 | GSE222962 | GEO