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In vivo evidence for ATPase-dependent DNA translocation by the Bacillus subtilis SMC condensin complex

ABSTRACT: Structural maintenance of chromosomes (SMC) complexes shape the genomes of virtually all organisms but how they function remains incompletely understood. Recent studies in bacteria and eukaryotes have led to a unifying model in which these ring-shaped ATPases act along contiguous DNA segments processively enlarging DNA loops. In support of this model, single-molecule imaging experiments indicate that Saccharomyces cerevisiae condensin complexes can extrude DNA loops in an ATP hydrolysis dependent manner in vitro. Here, using time-resolved high-throughput chromosome conformation capture (Hi-C) we investigate the interplay between ATPase activity of the Bacillus subtilis SMC complex and loop formation in vivo. We show that point mutants in the SMC nucleotide binding domain that impair but do not eliminate ATPase activity not only exhibit delays in de novo loop formation but also have reduced rates of processive loop enlargement. These data provide in vivo evidence that SMC complexes function as loop extruders.

ORGANISM(S): Bacillus subtilis PY79

PROVIDER: GSE95137 | GEO | 2018/08/09

REPOSITORIES: GEO

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Bacillus subtilis SMC complexes juxtapose chromosome arms as they travel from origin to terminus

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DNA-loop extruding SMC complexes can traverse one another in vivo

Project description:DNA-loop extruding SMC complexes can traverse one another in vivo

| PRJNA649231 | ENA

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