Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profile of neurons expressing protein kinase C δ (PKCδ) or somatostatin (SST) in the central amygdala (CEA) of mice

ABSTRACT: Circuit neuroscience has made great progress by linking neuronal function to marker gene expression, allowing the specific investigation of otherwise indistinguishable neuronal ensembles. Here, we performed next generation sequencing on two functionally and genetically distinct interneuronal populations marked by the expression of protein kinase C δ (PKCδ) or somatostatin (SST) in the central amygdala (CEA) of mice, which are known to play distinct and sometimes opposing roles in emotion processing. Making their gene expression profile known will aid in forming hypotheses of how different neurotransmitters or psychoactive drugs could alter information processing in these neurons.

ORGANISM(S): Mus musculus

PROVIDER: GSE95154 | GEO | 2018/12/26

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

The human brain somatostatin interactome: SST binds selectively to P-type family ATPases.

Project description: Not available

| S-EPMC6538167 | biostudies-literature

Genomewide effects of regular caffeine intake on hippocampal metabolism and learning-dependent transcription [Cut & Tag]

Project description:Caffeine is the most widely consumed psychoactive substance worldwide. Strikingly, molecular pathways engaged by its regular consumption remain unclear. We herein addressed the mechanisms associated with habitual (chronic) caffeine consumption in the mouse hippocampus using untargeted orthogonal-omics techniques. Our results revealed that chronic caffeine exerts concerted pleiotropic effects in the hippocampus, at the epigenomic, proteomic and metabolomic levels, lowered metabolic-related processes in bulk tissue, while inducing neuronal-specific epigenetic changes at synaptic transmission/neuronal activity-related genes. Altogether, these findings suggest that regular intake of caffeine improves the signal-to-noise ratio during information encoding in learning in part through a re-setting of metabolic genes helping to bolster the salience of information processing in neuronal circuits.

2022-06-22 | GSE200156 | GEO

Project description:An in vitro model of neuronal ensembles

| PRJNA832747 | ENA

DOTA-Derivatives of Octreotide Dicarba-Analogs with High Affinity for Somatostatin sst<sub>2,5</sub> Receptors.

Project description: Not available

| S-EPMC5324734 | biostudies-literature

Protein Kinase C Delta Regulates Mononuclear Phagocytes by Repressing Type I and II Interferon Signaling and Hinders Response to Immunotherapy in Cancer

Project description:Mononuclear phagocytes (MPs) comprise monocytes, macrophages and dendritic cells and play a crucial role in tissue homeostasis. However, accumulating evidence suggests that mononuclear phagocytes contribute to tumor progression and resistance to checkpoint blockade. Protein kinase C delta (PKCδ) is a serine/threonine kinase that plays a crucial role in regulating the immune response by preventing autoimmunity. However, the role of PKCδ in antitumor immunity is unknown. In this study, we found that PKCδ is abundantly expressed by MPs in several human and mouse tumors. PKCδ-/- mice were more resistant to growth of various cancers compared to wild-type mice and were more responsive to anti-PD-1 immunotherapy. Furthermore, we found that tumors from PKCδ-/- mice harbor a Th-1-skewed immune response including increased antigen cross-presentation and T cell activation. Depletion of MPs in vivo altered tumor growth in wild-type mice, but not in PKCδ-/- mice. In addition, co-injection of PKCδ-/- M2-like macrophages with cancer cells into wild-type mice markedly delayed tumor growth and significantly increased intratumoral T cell activation compared to wild-type M2-like macrophages co-injected with cancer cells. Finally, intrinsic loss of PKCδ functionally reprogrammed macrophages and dendritic cells by promoting their antigen presenting and cross-presenting capacity and triggered Type I and II interferon signaling. Our results suggest that PKCδ can be targeted to reprogram mononuclear phagocytes and augment checkpoint blockade efficacy.

2022-10-01 | GSE208394 | GEO

PKCδ regulates the transformed growth of K-ras dependent lung cancer cells through regulation of integrin αVβ3 expression

Project description:To explore how PKCδ regulates tumorigenesis, we performed mRNA expression analysis of four KRAS mutant NSCLC cell lines that stably express scrambled shRNA or PKCδ targeted shRNA

2016-09-01 | GSE72788 | GEO

PatchSeq experiment on somatostatin-expressing (Sst) neurons from mouse ventral tegmental area (VTA). Full-length single cell sequencing.

Project description:Experiment was done as a part of multi-methodological description of Sst-neurons subtypes in mouse VTA, aiming to reveal their transcriptional profiles and neurotransmitter nature.

2020-06-02 | E-MTAB-8780 | biostudies-arrayexpress

RNA sequencing from neural ensembles activated during fear conditioning in the mouse temporal association cortex

Project description:The stable formation of remote fear memories is thought to require neuronal gene induction in cortical ensembles that are activated during learning. However, the set of genes expressed specifically in these activated ensembles is not known; knowledge of such transcriptional profiles may offer insights into the molecular program underlying stable memory formation. Here we use RNA-Seq to identify genes whose expression is enriched in activated cortical ensembles labeled during associative fear learning. We first establish that mouse temporal association cortex (TeA) is required for remote recall of auditory fear memories. We then perform RNA-Seq in TeA neurons that are labeled by the activity reporter Arc-dVenus during learning. We identify 944 genes with enriched expression in Arc-dVenus+ neurons. These genes include markers of L2/3, L5b, and L6 excitatory neurons but not glial or inhibitory markers, confirming Arc-dVenus to be an excitatory neuron-specific, layer non-specific activity reporter. Cross comparisons to other transcriptional profiles show that 125 of the enriched genes are also activity-regulated in vitro or induced by visual stimulus in the visual cortex, suggesting that they may be induced generally in the cortex in an experience-dependent fashion. Prominent among the enriched genes are those encoding potassium channels that down-regulate neuronal activity, suggesting the possibility that part of the molecular program induced by fear conditioning may initiate homeostatic plasticity.

2016-08-31 | GSE85128 | GEO

Central amygdala cell types and their transcriptional regulation by fasting

Project description:A complete cell type census based on their full molecular profiles at subclass level and their subnuclei distribution in the central amygdala (CeA), linked with their behavior relevance, is still lacking. Here, we performed single nuclei RNA sequencing in adult mice and compared the results with known genetic and functionally defined populations. We identified 9 transcriptionally and spatially discrete cell types (3 in the lateral division CeL; 5 in the medial division CeM; and 1 in the capsular division CeC). In the CeL, we found one PKCδ expressing cluster (CeL PKCδ ) and two clusters marked by Sst: the CeL Sst cluster which contains the largest and most highly expressing population of Sst-positive neurons, and the CeL NTS/Tac2 cluster which contains low expressing Sst-positive cells. This two Sst population are also Htr2a positive. The CeC contains one cluster marked by the expression of Calcrl and partially expressed PKCδ. In the CeM, the largest cluster was marked by Il1Rapl2, which contains the CeM subsets of Pnoc and Htr2a, and is likely appetitive. Another Sst population, the CeM Tac1/Sst, remains largely unexplored. Moreover, we discovered three previously uncharacterized CeM clusters: CeM Vdr, CeM Drd2/Rai14, and CeM Dlk1. To begin investigating whether these cell types show individual responses in appetitive behavior, we compared their transcriptomes in food-deprived and satiated mice. We found a cell type specific transcriptional response across neuronal populations, particularly in one of the appetitive clusters in the CeL.

2023-05-06 | GSE231790 | GEO

Microarray and Pathway Analysis Reveal Distinct Mechanisms Underlying Cannabinoid-Mediated Modulation of LPS-Induced Activation of BV-2 Microglial Cells

Project description:Cannabinoids are known to exert immunosuppressive activities. However, the mechanisms which contribute to these effects are unknown. Using lipopolysaccharide (LPS) to activate BV-2 microglial cells, we examined how Δ9-tetrahydrocannabinol (THC), the major psychoactive component of marijuana, and cannabidiol (CBD) the non-psychoactive component, modulate the inflammatory response.

2015-07-09 | GSE70689 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data