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Empirical comparison of reduced representation bisulfite sequencing and Infinium Beadchip reproducibility and coverage of DNA methylation in humans

ABSTRACT: We empirically examined the strength of rapid multiplexed reduced representation bisulfite sequencing (rmRRBS) and Illumina’s Infinium Beadchip. rmRRBS required less input DNA, offered more flexibility in coverage, and interrogated more CpG loci at a higher regional density. The Infinium covered slightly more protein coding, cancer-associated and mitochondrial-related genes, both platforms covered all known imprinting clusters, and rmRRBS covered more microRNA genes than the HumanMethylation450 but fewer than the MethylationEPIC. rmRRBS did not always interrogate exactly the same CpG loci, but genomic tiling improved overlap between different libraries.

ORGANISM(S): Homo sapiens

PROVIDER: GSE95163 | GEO | 2017/02/22

SECONDARY ACCESSION(S): PRJNA376214

REPOSITORIES: GEO

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