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Thy1+ 2dpp gonocytes RA regulation

ABSTRACT: to investigate the RA regulated genes in 2 dpp thy1+ gonocytes Keywords: RA effects on gonocytes

ORGANISM(S): Mus musculus

PROVIDER: GSE9521 | GEO | 2007/11/07

SECONDARY ACCESSION(S): PRJNA103325

REPOSITORIES: GEO

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Transcription profiling of mouse gonocytes isolated from 2 dpp mouse testes by THY1+ MACS sorting and treated with retinoic acid

Project description:to investigate the RA regulated genes in 2 dpp thy1+ gonocytes Experiment Overall Design: gonocytes were isolated from 2 dpp mouse testes by THY1+ MACS sorting and cultured without serum, growth factors and feeder cells for 8hr, with either vehicle ethanol or RA (100 nM). Two control and two RA treated samples were included.

2008-06-16 | E-GEOD-9521 | biostudies-arrayexpress

Mus musculus

Project description:Thy1+ 2dpp gonocytes RA regulation

| PRJNA103325 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of germ cell RA signaling mutant and control THY1+ spermatogonia Transcriptomes

Project description:Purpose: The goal of this sequencing is to investigate alterations in gene expression that result from impaired retinoid signaling compared with control, and how the RA signaling controls spermatogonia differentiation Methods: THY1+ spermatogonia mRNA profiles of 4-day-old control and germ cell specific impaired retinoid signaling mice were generated by High-throughput sequencing Results: Gene ontology (GO) analysis of the genes at the top of the ranked genes indicated enrichment in genes associated with roles in reproduction, transcription and spermatogenesis. In total, we identified 1633 and 742 transcripts (Reads Per Kilobase of transcript per Million mapped reads (RPKM) > 1) that were significantly (p-value < 0.05, > 1.5-fold difference) down- and up-regulated, respectively, in the germ cell mutants compared with the controls. Most importantly, we found that the majority of transcripts of replication-dependent core histone genes, histone cluster 1 (Hist1) were downregulated in germ cell mutants. THY1+ spermatogonia mRNA profiles of 4-day old germ cell specific impaired retinoid signaling and control mice were generated by deep sequencing, twice, using Illumina HiSeq 2000

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Next Generation Sequencing Facilitates Quantitative Analysis of germ cell RA signaling mutant and control THY1+ spermatogonia Transcriptomes

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Gene regulation by phosphomimetic Myc (MycD), cytosine arabinoside (AraC) and retinoic acid (RA)

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Gene Expression Profiling of the SSC-Enriched Thy1+ and SSC-Depleted Thy1- Fractions of Prepubertal Mouse Testes

Project description:Self-renewal and differentiation of spermatogonial stem cells (SSCs) provides the foundation for testis homeostasis, yet mechanisms that control their functions in mammals are poorly defined. We used microarray transcript profiling to identify specific genes whose expression are augmented in the SSC-enriched Thy1+ germ cell fraction of mouse pup testes. Comparisons of gene expression in the Thy1+ germ cell fraction to the Thy1-depeleted testis cell population identified 202 genes that are expressed 10-fold or higher in Thy1+ cells. This database provided a mining tool to investigate specific characteristics of SSCs and identify novel mechanisms that potentially influence their functions.

2008-12-30 | GSE14222 | GEO

Gene regulation by phosphomimetic Myc (MycD), cytosine arabinoside (AraC) and retinoic acid (RA)

Project description:We aimed to investigate whether the previously observed synergistic effects of AraC and RA in stimulating leukemia differentiation could be at least partially dependent on Pak2-mediated phosphorylation of Myc. Genome-wide analysis of NB4 cells treated with RA, AraC with RA, or MycD with RA revealed a significant 60% overlap between RA-target genes superactivated by AraC with RA, or MycD with RA, with respect to RA alone. NB4 cells infected with ER-tagged inducible empty vector (CTL), MycD or MycA were treated with 4-OHT (200 nM, 16 h) to induce MycD-ER and MycA-ER expression. CTL cells were either vehicle or AraC (25 μM) treated for 16 h. Short treatment of RA (10 nM, 4 h) was performed in CTL, AraC and MycD-expressing cells. Four biologically independent experiments were performed at each time.

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