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KDM4 inhibition targets breast cancer stem cells [RNA-Seq]

ABSTRACT: We treated breast cancer stemm cells (BCSC) with a KDM4 inhibitor

ORGANISM(S): Homo sapiens

PROVIDER: GSE95293 | GEO | 2017/12/15

SECONDARY ACCESSION(S): PRJNA376575

REPOSITORIES: GEO

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KDM4 inhibition targets breast cancer stem cells [ChIP-Seq]

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KDM4 inhibition targets breast cancer stem cells

Project description:Cancer progression is associated with alterations of epigenetic regulators such as histone-lysine demethylases 4 (KDM4)2-5. During breast cancer therapy, classical treatments fail to address resistant cancer stem cell populations6-10. Here, we identified a novel KDM4 inhibitor (KDM4(i)) with unique preclinical characteristics. KDM4(i) is a highly potent pan KDM4 inhibitor that specifically blocks the demethylase activity of KDM4A, B, C, and D but not that of the other members of the KDM family. We validated the KDM4(i) anti-tumoral properties under conditions recapitulating patient tumors. Therefore, we established a method to isolate and grow triple-negative breast cancer stem cells (BCSCs) from individual patient tumors after neoadjuvant chemotherapy. Limiting dilution orthotopic xenografts of these BCSCs faithfully regenerate original patient tumor histology and gene expression. KDM4(i) blocks proliferation, sphere formation and xenograft tumor growth of BCSCs. Importantly, KDM4(i) abrogates expression of EGFR, a driver of therapy-resistant triple-negative breast tumor cells11, via inhibition of the KDM4A demethylase activity. Taken together, we present a unique BCSC culture system as a basis for therapeutic compound identification and demonstrate that KDM4 inhibition is a new therapeutic strategy for the treatment of triple-negative breast cancer.

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KDM4 inhibition targets breast cancer stem cells

Project description:KDM4 inhibition targets breast cancer stem cells

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KDM4 inhibition targets breast cancer stem cells

Project description:This SuperSeries is composed of the SubSeries listed below.

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Expression data from tumor tissue-derived long-term-cultured breast cancer stem cells

Project description:Here we established two tumor tissue-derived long-term-cultured breast cancer stem cells (BCSC). RNA extraction and microarray analysis total RNA from BCSC treating with G1, G15, or control (Ctrl) for 48 hr were extracted using Trizol Reagent. Important significant differences of cDNA genes were identified. (The G1-treated group was designed as \\"12\\", the control group was \\"11\\" (raw data: 11 and 12; processed data: BCSC-G1). Similarly, the G15-treated study group was defined as \\"14\\", the control group was \\"13\\" (raw data: 13 and 14; processed data: BCSC-G15).

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