Transcriptomics,Genomics

Dataset Information

48

Transcriptomic changes in response to different carbon sources in Neurospora crassa


ABSTRACT: Purpose: We explore gene expression changes when Neurospora crassa wild type responds to different carbon sources in Vogel's medium. Method: We obtained mRNA samples of Neurospora crassa WT in Vogel's minimal medium (VMM) with different carbon source and used RNA-seq technique to measure the trancriptome changes. Results: We identified many genes of transcription factors and enzymes that were up regulated or down regulated in response to the different carbon stimulation. Conclusion: Our data represents a systematic transcriptome profiling of filamentous fungi on different carbon source and identify COL-26 as a critical regulator in degradation of starch components. Overall design: mRNA profiles of cultures of WT after 4 hours of exposure to media of interest were generated by deep sequencing, in triplicate, using Illumina HiSeq2000. Soluble carbon sources are 2 mM and insoluble carbon source are 2% (w/v).

INSTRUMENT(S): Illumina HiSeq 2000 (Neurospora crassa)

SUBMITTER: Yi Xiong  

PROVIDER: GSE95350 | GEO | 2017-04-09

SECONDARY ACCESSION(S): PRJNA376729

REPOSITORIES: GEO

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Publications

A fungal transcription factor essential for starch degradation affects integration of carbon and nitrogen metabolism.

Xiong Yi Y   Wu Vincent W VW   Lubbe Andrea A   Qin Lina L   Deng Siwen S   Kennedy Megan M   Bauer Diane D   Singan Vasanth R VR   Barry Kerrie K   Northen Trent R TR   Grigoriev Igor V IV   Glass N Louise NL  

PLoS genetics 20170503 5


In Neurospora crassa, the transcription factor COL-26 functions as a regulator of glucose signaling and metabolism. Its loss leads to resistance to carbon catabolite repression. Here, we report that COL-26 is necessary for the expression of amylolytic genes in N. crassa and is required for the utilization of maltose and starch. Additionally, the Δcol-26 mutant shows growth defects on preferred carbon sources, such as glucose, an effect that was alleviated if glutamine replaced ammonium as the pr  ...[more]

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