Transcriptomics,Genomics

Dataset Information

49

RNA expression analysis in RNA Pol II CTD mutants


ABSTRACT: The carboxyl-terminal domain (CTD) of the largest subunit of RNA polymerase II (Pol II) orchestrates dynamic recruitment of specific cellular machines during different stages of transcription. Signature phosphorylation patterns of Y1S2P3T4S5P6S7 heptapeptide repeats of the CTD engage specific “readers.” While phospho-Ser5 and phospho-Ser2 marks are ubiquitous, phospho-Thr4 is reported to only impact specific genes. Here, we investigate the RNA expression profile in WT and CTD-mutant strains of S. cerevisiae. Overall design: RNA expression analysis in RNA Pol II CTD mutants

INSTRUMENT(S): S. cerevisiae 2006-10-12_Ansari_tiling_51mer

SUBMITTER: Aseem Ansari 

PROVIDER: GSE95417 | GEO | 2017-05-02

SECONDARY ACCESSION(S): PRJNA377087

REPOSITORIES: GEO

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Publications

Different phosphoisoforms of RNA polymerase II engage the Rtt103 termination factor in a structurally analogous manner.

Nemec Corey M CM   Yang Fan F   Gilmore Joshua M JM   Hintermair Corinna C   Ho Yi-Hsuan YH   Tseng Sandra C SC   Heidemann Martin M   Zhang Ying Y   Florens Laurence L   Gasch Audrey P AP   Eick Dirk D   Washburn Michael P MP   Varani Gabriele G   Ansari Aseem Z AZ  

Proceedings of the National Academy of Sciences of the United States of America 20170502 20


The carboxyl-terminal domain (CTD) of the largest subunit of RNA polymerase II (Pol II) orchestrates dynamic recruitment of specific cellular machines during different stages of transcription. Signature phosphorylation patterns of Y1S2P3T4S5P6S7 heptapeptide repeats of the CTD engage specific "readers." Whereas phospho-Ser5 and phospho-Ser2 marks are ubiquitous, phospho-Thr4 is reported to only impact specific genes. Here, we identify a role for phospho-Thr4 in transcription termination at nonco  ...[more]

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