Genomics

Dataset Information

160

Assessing the impact of loss of MORC2 and SETDB1 on the global distribution of H3K9me3


ABSTRACT: By comparing HeLa cells lacking MORC2 or SETDB1 generated through CRISPR/Cas9-mediated gene disruption to wild-type HeLa cells, the goal of the experiment was to determine the effect of loss of MORC2 on the distribution of the repressive H3K9me3 histone modification. Overall design: H3K9me3 ChIP-seq analysis of wild-type HeLa cells compared to MORC2 knockout and SETDB1 knockout HeLa cells.

INSTRUMENT(S): Illumina HiSeq 2500 (Homo sapiens)

SUBMITTER: Iva Tchasovnikarova  

PROVIDER: GSE95456 | GEO | 2017-05-29

SECONDARY ACCESSION(S): PRJNA377248

REPOSITORIES: GEO

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Publications

Hyperactivation of HUSH complex function by Charcot-Marie-Tooth disease mutation in MORC2.

Tchasovnikarova Iva A IA   Timms Richard T RT   Douse Christopher H CH   Roberts Rhys C RC   Dougan Gordon G   Kingston Robert E RE   Modis Yorgo Y   Lehner Paul J PJ  

Nature genetics 20170605 7


Dominant mutations in the MORC2 gene have recently been shown to cause axonal Charcot-Marie-Tooth (CMT) disease, but the cellular function of MORC2 is poorly understood. Here, through a genome-wide CRISPR-Cas9-mediated forward genetic screen, we identified MORC2 as an essential gene required for epigenetic silencing by the HUSH complex. HUSH recruits MORC2 to target sites in heterochromatin. We exploited a new method, differential viral accessibility (DIVA), to show that loss of MORC2 results in  ...[more]

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