Transcriptomics,Genomics

Dataset Information

22

Zea mays Transcriptome or Gene expression


ABSTRACT: The experiment consisted of a field design containing two maize inbred lines Va35 and Mp313E. Va35 has yellow kernels and is susceptible to kernel infection by Aspergillus flavus (A. flavus). Mp313E is a white dent maize inbred line and was released primarily as a source of resistance to kernel infection by A. flavus. The test ears were inoculated with Aspergillus flavus and collected two days after inoculation. Non-inoculated ears collected 16 days after pollination were also used as a control. The microarray experimental design was a randomized complete block design with three replications. One microarray slide was used for each field plot. Each slide contained the inoculated and non-inoculated sub-treatments. A second slide for one replication of each genotype contained a dye swap for the inoculation treatment and three subsamples (dots) within the slide represented each contig. Keywords: Direct comparison Overall design: Two-condition experiment, inoculated vs. uninoculated maize ears for Va35 and Mp313E. Biological replicates: 3 control, 3 inoculated, 2 dye-swap, independently grown and harvested. One replicate per array.

INSTRUMENT(S): MGDP Zea mays Unigene 01_01_05

SUBMITTER: Rowena Yvette Kelley     

PROVIDER: GSE9546 | GEO | 2007-11-09

SECONDARY ACCESSION(S): PRJNA103361

REPOSITORIES: GEO

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Publications


BACKGROUND: Aspergillus flavus Link:Fr, an opportunistic fungus that produces aflatoxin, is pathogenic to maize and other oilseed crops. Aflatoxin is a potent carcinogen, and its presence markedly reduces the value of grain. Understanding and enhancing host resistance to A. flavus infection and/or subsequent aflatoxin accumulation is generally considered an efficient means of reducing grain losses to aflatoxin. Different proteomic, genomic and genetic studies of maize (Zea mays L.) have generate  ...[more]

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