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Structure-seq2: Sensitive and accurate genome-wide profiling of RNA structure in vivo

ABSTRACT: We present Structure-seq2, which provides nucleotide-resolution RNA structural information in vivo and genome-wide. This optimized version of our original Structure-seq method increases sensitivity and data quality by minimizing formation of a deleterious by-product, reducing ligation bias, and improving read coverage. Structure-seq2 can employ a biotinylated nucleotide to facilitate the protocol. We have benchmarked Structure-seq2 on both mRNA and rRNA structure in rice (Oryza sativa) and apply Structure-seq2 to provide evidence of hidden breaks in chloroplast rRNA and a previously unreported N1-methyladenosine (m1A) in a nuclear-encoded rRNA.

ORGANISM(S): Oryza sativa Japonica Group

PROVIDER: GSE95514 | GEO | 2017/06/08

SECONDARY ACCESSION(S): PRJNA377316

REPOSITORIES: GEO

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Project description: Not available

| S-EPMC5737731 | biostudies-literature

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