Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional response to conditional over-expression of GDV1 in Plasmodium falciparum 3D7 wild type and F12 mutant parasites

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

OTHER RELATED OMICS DATASETS IN: PRJNA377430PRJNA377421PRJNA377429PRJNA374799

ORGANISM(S): Plasmodium falciparum Plasmodium falciparum 3D7

PROVIDER: GSE95549 | GEO | 2017/03/02

SECONDARY ACCESSION(S): PRJNA377421

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Transcriptional response to conditional over-expression of GDV1 in Plasmodium falciparum 3D7 wild type and F12 mutant parasites

Project description:Transcriptional response to conditional over-expression of GDV1 in Plasmodium falciparum 3D7 wild type and F12 mutant parasites

| PRJNA377421 | ENA

Transgenic Plasmodium falciparum blood stage parasites F12/GDV1-GFP-DD: control (-Shield-1;F12/GDV1-GFP-DDOFF) vs GDV1-overexpressing (+Shield-1;F12/GDV1-GFP-DDON) parasites

Project description:Transcriptional profiling of transgenic P. falciparum blood stage parasites expressing an ectopic copy of GDV1-GFP-DD (F12/GDV1-GFP-DD). The DD (FKBP destabilisation domain) allows for the conditional expression of fusion proteins: DD fusion proteins are rapidly degraded or stably expressed in absence or presence of the stabilising ligand Shield-1, respectively (Banaszynski LA, Chen LC, Maynard-Smith LA, Ooi AG, Wandless TJ. A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules.Cell. 2006 Sep 8;126(5):995-1004). The goal of this experiment was to identify genes differentially expressed in response to induced GDV1 overexpression.

2017-03-02 | GSE95548 | GEO

Expression analysis of Plasmodium falciparum 3D7 delta-PfPuf2 mutant

2012-07-06 | GSE39124 | GEO

Artemisinin activity-based probes identify multiple molecular targets within the asexual stage of the malaria parasites Plasmodium falciparum 3D7.

Project description: Not available

| S-EPMC4776496 | biostudies-literature

RNAseq of Plasmodium falciparum 3D7 parasites treated with the Jumonji pan inhibitor JIB-04

Project description:Little is known about the role of the three Jumonji C (JmjC) enzymes in Plasmodium falciparum (Pf). Here, we show that JIB-04 and other established inhibitors of mammalian JmjC histone demethylases kill asexual blood stage parasites and are even more potent at blocking gametocyte development and gamete formation. In late stage parasites, JIB-04 increased levels of trimethylated lysine residues on histones, suggesting the inhibition of P. falciparum Jumonji demethylase activity. These epigenetic defects coincide with deregulation of invasion, cell motor, and sexual development gene programs, including gene targets coregulated by the PfAP2-I transcription factor and chromatin-binding factor, PfBDP1. Mechanistically, we demonstrate that PfJmj3 converts 2-oxoglutarate to succinate in an iron-dependent manner consistent with mammalian Jumonji enzymes, and this catalytic activity is inhibited by JIB-04 and other Jumonji inhibitors. Our pharmacological studies of Jumonji activity in the malaria parasite provide evidence that inhibition of these enzymatic activities is detrimental to the parasite.

2020-07-12 | GSE117307 | GEO

Expression analysis of Plasmodium falciparum 3D7 delta-PfPuf2 mutant

Project description:Investigation of whole genome gene expression level changes in Plasmodium falciparum 3D7 delta-PfPuf2 mutant, compared to the wild-type strain 3D7. The mutation engineered into this strain render tanslational control. The mutants analyzed in this study are further described in Miao J, Li J, Fan Q, Li X, Li X, Cui L.2010. The Puf-family RNA-binding protein PfPuf2 regulates sexual development and sex differentiation in the malaria parasite Plasmodium falciparum. J Cell Sci. 123(7):1039-49 (PMID 20197405). A 12 chip study using total RNA recovered from six separate wild-type cultures of Plasmodium falciparum 3D7 at gametocyte stage III (three cultures) and stage V (three cultures) and six separate cultures of dalta PfPuf2 mutant at gametocyte stage III (three cultures) and stage V (three cultures). Each chip measures the expression level of 5,367 genes from Plasmodium falciparum 3D7 with 45-60 mer probes with two replicates on final array of 71618 probes.

2012-07-05 | E-GEOD-39124 | biostudies-arrayexpress

Identification and characterization of DNA endonucleases in Plasmodium falciparum 3D7 clone.

Project description: Not available

| S-EPMC6006590 | biostudies-literature

Antifolate Agents Against Wild and Mutant Strains of Plasmodium falciparum.

Project description: Not available

| S-EPMC4023280 | biostudies-literature

A resistant mutant of Plasmodium falciparum purine nucleoside phosphorylase uses wild-type neighbors to maintain parasite survival.

Project description: Not available

| S-EPMC7949152 | biostudies-literature