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Hemicastration induced spermatogenesis related DNA methylation and gene expression changes in mice testis

ABSTRACT: Hemicastration is unilateral orchiectomy to remove an injured testis, and may influence spermatogenesis. However, the underlying molecular mechanisms are poorly understood. Here, we monitored hemicastrated mice and found no significant difference of growth or testosterone (P > 0.05) compared with control. The genome-wide DNA methylation pattern of remaining testis were investigated by RRBS. Substantial genes harbored differentially methylated regions in gene bodies, and were enriched in process of protein binding and cell adhesion. Moreover, RNA-seq results indicated that 46 DEGs involved in meiotic cell cycle, synaptonemal complex assembly and spermatogenesis were upregulated in the hemicastration group, while 197 DEGs were downregulated, which were related to arachidonic acid metabolism. Integrative analysis revealed that PSMC3IP, which encodes a protein crucial for homologous recombination in spermatocytes, exhibited promoter hypomethylation and higher expression level in hemicastrated mice. Our results demonstrated that hemicastration-induced compensatory response maintained normal growth and fertility in mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE95693 | GEO | 2018/01/24

REPOSITORIES: GEO

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Hemicastration induced spermatogenesis-related DNA methylation and gene expression changes in mice testis

Project description:Hemicastration is unilateral orchiectomy to remove an injured testis, and may influence spermatogenesis. However, the underlying molecular mechanisms are poorly understood. Here, we monitored hemicastrated mice and found no significant difference of growth or testosterone (P > 0.05) compared with control. The genome-wide DNA methylation pattern of remaining testis were investigated by RRBS. Substantial genes harbored differentially methylated regions in gene bodies, and were enriched in process of protein binding and cell adhesion. Moreover, RNA-seq results indicated that 46 DEGs involved in meiotic cell cycle, synaptonemal complex assembly and spermatogenesis were upregulated in the hemicastration group, while 197 DEGs were downregulated, which were related to arachidonic acid metabolism. Integrative analysis revealed that PSMC3IP, which encodes a protein crucial for homologous recombination in spermatocytes, exhibited promoter hypomethylation and higher expression level in hemicastrated mice. Our results demonstrated that hemicastration-induced compensatory response maintained normal growth and fertility in mice.

2018-01-24 | GSE95692 | GEO

Glis3 plays a crucial role in spermatogenesis [923]

Project description:We show that Glis3 is expressed in gonocytes, SSCs and SPCs, but not in differentiated spermatogonia or subsequent stages of spermatogenesis nor in Sertoli or Leydig cells. We further demonstrate that Glis3-deficiency causes a severe impairment in spermatogenesis in mice. Although the number of gonocytes was slightly diminished in Glis3KO testis, the number undifferentiated, PLZF+ spermatogonia was dramatically reduced leading to a virtual block in the progression of spermatogenesis. Gene expression profiling showed that the expression of a number of genes associated with self-renewal and differentiation of spermatogonial cells was significantly decreased in 1-week-old Glis3KO2 testis. These included a set of GDNF-dependent genes, such as Etv5, Bcl6b, Lhx1, Brachyury, Id4, and Pou3f1, and GDNF-independent genes, such as FoxO1, Oct4, and Zbtb16. Impairment of the nuclear localization of FoxO1 may be in part responsible for the reduced expression of Ret, Lhx1, and Sall4 in Glis3KO2 testis. Our study identifies Glis3 as a novel and critical regulator of early stages of spermatogenesis. Thy1+ cells were isolated from 3 WT and 3 Glis3KO2 testis at postnatal day 4, and total RNAs were purified from them. Then the samples were applied to Agilent mouse genome chip.

2016-07-01 | E-GEOD-70195 | biostudies-arrayexpress

Glis3 plays a crucial role in spermatogenesis [848_827]

Project description:We show that Glis3 is expressed in gonocytes, SSCs and SPCs, but not in differentiated spermatogonia or subsequent stages of spermatogenesis nor in Sertoli or Leydig cells. We further demonstrate that Glis3-deficiency causes a severe impairment in spermatogenesis in mice. Although the number of gonocytes was slightly diminished in Glis3KO testis, the number undifferentiated, PLZF+ spermatogonia was dramatically reduced leading to a virtual block in the progression of spermatogenesis. Gene expression profiling showed that the expression of a number of genes associated with self-renewal and differentiation of spermatogonial cells was significantly decreased in 1-week-old Glis3KO2 testis. These included a set of GDNF-dependent genes, such as Etv5, Bcl6b, Lhx1, Brachyury, Id4, and Pou3f1, and GDNF-independent genes, such as FoxO1, Oct4, and Zbtb16. Impairment of the nuclear localization of FoxO1 may be in part responsible for the reduced expression of Ret, Lhx1, and Sall4 in Glis3KO2 testis. Our study identifies Glis3 as a novel and critical regulator of early stages of spermatogenesis. Testis total RNAs were purified from 4 WT and 4 Glis3KO2 at 1 week old age, and 3WT and 3 Glis3KO2 at 3 week-old age. Then the samples were applied to Agilent mouse genome chip.

2016-07-01 | E-GEOD-70194 | biostudies-arrayexpress

Glis3 plays a crucial role in spermatogenesis [923]

2016-07-01 | GSE70195 | GEO

Glis3 plays a crucial role in spermatogenesis [848_827]

2016-07-01 | GSE70194 | GEO

Transcriptional profiling of flatfish (Solea senegalensis) spermatogenesis: mid versus late spermatogenesis

Project description:The Senegalese sole (Solea senegalensis) is a marine flatfish of high economic value and a target species for aquaculture. Here, we used a transcriptomic approach to investigate changes in genes expressed in the Senegalese sole testis throughout spermatogenesis in wild-caught fish adapted to captivity. We identified approximately 400 genes that are differentially expressed during the progression of spermatogenesis and that participate in processes such as activation of the ubiquitin-proteasome system, sperm maturation and motility, cell adhesion or cytoskeletal remodeling. The results from this study contribute to our understanding of the molecular changes ocurring during spermatogenesis in the Senegalese sole.

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Transcriptional profiling of flatfish (Solea senegalensis) spermatogenesis: early versus late spermatogenesis

2010-05-07 | GSE21634 | GEO

Transcriptional profiling of flatfish (Solea senegalensis) spermatogenesis: functional mature versus late spermatogenesis

2010-05-07 | GSE21635 | GEO

Transcription profiling of spermatogenesis in juvenile mouse testis and analysis of four germ cell deficient mouse models, time series

Project description:Temporal study of the first wave of spermatogenesis in juvenile mouse testis and analysis of four germ cell deficient mouse models.<br> A series of purified testis cell-types (McCarrey libraries)constituting a focussed gene set was exploited to examine gene expression in prepubertal mouse testis. Testis RNA from C57/BL6J mice at various ages post partum was compared to a common control consisting of adult (8 week) testis RNA from the same strain(also corresponding to the last time-point).This generates a time course showing the activation of genes on the array across the first wave of spermatogenesis, which can then be correlated with the appearance of specific germ cell types, allowing assignation of unknown genes to differing cell types. <br> <br> Adult testis RNA from four different genetic models of infertility (XXSxrb, mshi, Bax -/-, bs) were compared to age- and strain-matched normal control testis RNA. These models possess different cellular complements within the testis, which can be interpreted within the framework established by the first wave analysis and used to refine the assignment of genes to cell types.

2006-08-10 | E-MEXP-571 | biostudies-arrayexpress

Transcription profiling of Drosophila melanogaster spermatogenesis: mitotic, meiotic and post-meiotic cells

Project description:We conducted a genome-wide expression analysis of wild-type males using three cell populations isolated from mitotic, meiotic and post-meiotic phases of spermatogenesis in Drosophila melanogaster. Our approach was to directly isolate testis regions enriched with RNAs from each of the three specific germline phases. We used microarrays to detail the global gene expression profile in spermatogenesis and identified up- and down-regulated genes between two different spermatogenic phases in pairwise comparisons Experiment Overall Design: Cell types present at various stages of spermatogenesis are generally located in a gradient along the proximal-distal axis of the testis, however most are not exclusively restricted to any one geographic region. Cells enriched for mitotic, meiotic and post-meiotic phases were obtained by dissection of apical, proximal and distal regions of the testis, respectively

2009-11-28 | E-GEOD-18502 | biostudies-arrayexpress

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