Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effects of anti-complement component 5 antibody targeting MG4 domain on the normal retina

ABSTRACT: This study aims to investigate the effects of anti-complement component 5 antibody targeting MG4 domain on normal retina.

ORGANISM(S): Mus musculus

PROVIDER: GSE95742 | GEO | 2017/03/07

SECONDARY ACCESSION(S): PRJNA378248

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Anti-complement component 5 antibody targeting MG4 domain inhibits choroidal neovascularization.

Project description: Not available

| S-EPMC5542204 | biostudies-literature

Cellular milieu of the neural retina informs complement immune regulation

Project description:Cellular milieu of the neural retina informs complement immune regulation

| PRJNA483791 | ENA

Therapeutic Intervention with Anti-Complement Component 5 Antibody Does Not Reduce NASH but Does Attenuate Atherosclerosis and MIF Concentrations in Ldlr-/-.Leiden Mice.

Project description:Background: Chronic inflammation is an important driver in the progression of non-alcoholic steatohepatitis (NASH) and atherosclerosis. The complement system, one of the first lines of defense in innate immunity, has been implicated in both diseases. However, the potential therapeutic value of complement inhibition in the ongoing disease remains unclear. Methods: After 20 weeks of high-fat diet (HFD) feeding, obese Ldlr-/-.Leiden mice were treated twice a week with an established anti-C5 antibody (BB5.1) or vehicle control. A separate group of mice was kept on a chow diet as a healthy reference. After 12 weeks of treatment, NASH was analyzed histopathologically, and genome-wide hepatic gene expression was analyzed by next-generation sequencing and pathway analysis. Atherosclerotic lesion area and severity were quantified histopathologically in the aortic roots. Results: Anti-C5 treatment considerably reduced complement system activity in plasma and MAC deposition in the liver but did not affect NASH. Anti-C5 did, however, reduce the development of atherosclerosis, limiting the total lesion size and severity independently of an effect on plasma cholesterol but with reductions in oxidized LDL (oxLDL) and macrophage migration inhibitory factor (MIF). Conclusion: We show, for the first time, that treatment with an anti-C5 antibody in advanced stages of NASH is not sufficient to reduce the disease, while therapeutic intervention against established atherosclerosis is beneficial to limit further progression.

2022-09-19 | GSE195614 | GEO

Fc Gamma Receptors and Complement Component 3 Facilitate Anti-fVIII Antibody Formation.

Project description: Not available

| S-EPMC7295897 | biostudies-literature

Transcriptome of U251 cells overexpression complement component 7

Project description:We identified a rare coding variant (p.K420Q) in the complement component 7 (C7) gene affecting the risk of Alzheimer's disease. To investigate the cellular effects of the mutant, we performed RNA-seq in cell line overexpression wilt-type and mutant C7. U251 glioma cells with stable expression of mutant APP (K670N/M671L) (U251-APP cells), which produce Aβ42 under Dox inducing, were used as the model cell. Total RNA of U251-APP cells overexpressing wild type and mutant C7 proteins were subjected to transcriptome sequencing using Illumina Hiseq 4000 platform.

2018-11-22 | GSE101608 | GEO

Otx2 ChIP-seq in the adult mouse Retinal Pigmented Epithelium (RPE) and neural retina

Project description:Otx2 has been shown to be non cell autonomously required for photoreceptor cell survival in the adult mouse RPE. This study aims to identify Otx2 DNA binding profile in both RPE and neural retina to i) identify direct targets of Otx2 in the RPE ii) compare Otx2 binding profile in neural retina and RPE to unveil hidden functions in the neural retina. WT and GFP antibodies were used to perform two independent ChIP-seq experiments using Illumina GAIIx.

2014-01-15 | E-GEOD-54084 | biostudies-arrayexpress

Liver gene expression profiling of complement component 3a receptor 1 knockout (C3ar1-/-) mice

Project description:Complement component 3a receptor 1 (C3ar1) was predicted as a causal gene for abdominal using a novel statistical method named LCMS (Schadt et al., 2005, Nature Genetics). In order to validate this prediction, we profiled the liver tissues of complement component 3a receptor 1 knockout (C3ar1-/-) mice and their littermate wild-type (wt) controls to examine the gene expression signature as well as pathways/networks resulting from the single gene perturbation.

2009-03-08 | GSE11995 | GEO

Liver gene expression profiling of complement component 3a receptor 1 knockout (C3ar1-/-) mice

2009-03-07 | E-GEOD-11995 | biostudies-arrayexpress

Otx2 ChIP-seq in the adult mouse Retinal Pigmented Epithelium (RPE) and neural retina

2014-01-15 | GSE54084 | GEO

Anatomical-spatial dissection of single-cell retinal transcriptomics paves the road for new horizons in complement immunology [Mm]

Project description:Purpose: We present the first comprehensive map of the murine retinal complement expression at a single-cell resolution from ~92,000 retinal cells, spanning 11 cell types. Methods: Retinal mRNA profiles from 10-week-old wild-type (WT) Black 6 mouse eyes were generated by scRNA sequencing. The data was normalized using Seurat. Results: Using an optimized data analysis workflow, we found novel patterns of complement expression in the retina Conclusions: Our landmark atlas provides crucial mechanistic insights in complement immunology

2020-01-24 | GSE116426 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data