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Assessment of the Pol IV largest subunit, NRPD1, DeCL domain deletion construct to rescue Pol IV-dependent DNA methylation in the nrpd1-3 mutant (Bisulfite-Seq).

ABSTRACT: The Arabidopsis thaliana multi-subunit RNA Polymerase IV largest subunit DeCL domain was functionally assessed with an NRPD1 DeCL domain deletion construct.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE95823 | GEO | 2017/07/17

SECONDARY ACCESSION(S): PRJNA378528

REPOSITORIES: GEO

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Assessment of the Pol IV largest subunit, NRPD1, DeCL domain deletion construct to rescue Pol IV-dependent siRNAs in the nrpd1-3 mutant (sRNA).

Project description:The Arabidopsis thaliana multi-subunit RNA Polymerase IV largest subunit DeCL domain was functionally assessed with an NRPD1 DeCL domain deletion construct.

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Project description:Using MethylC-Seq to provide single-base resolution of DNA methylation status in nrpd1-3, nrpd1-12, and nrpe1-11 mutants

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Project description:Using MethylC-Seq to provide single-base resolution of DNA methylation status in nrpd1-3, nrpd1-12, and nrpe1-11 mutants MethylC-Seq: 3 mutants examined, nrpd1-3, nrpd1-12, and nrpe1-11

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Project description:Monocot grass species (Poaceae) express a diverse set of multisubunit RNA polymerase enzymes, including Pol II, Pol IV and Pol V. To better understand this functional diversity, we have charted Pol IV function in the model Brachypodium distachyon. Intriguingly, pol IV null mutations in Poaceae crops disrupt growth, reproductive development and seed set. In order to investigate how Pol IV controls vegetative growth and TE activity in these grasses, we have isolated B. distachyon mutant alleles for Pol IV’s largest subunit, NRPD1. We obtained the germplasm in which to screen for these pol IV mutations from the B. distachyon community's sodium azide (NaN) and T-DNA insertion collections.

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Arabidopsis thaliana

Project description:Assessment of the Pol IV largest subunit, NRPD1, DeCL domain deletion construct to rescue Pol IV-dependent siRNAs in the nrpd1-3 mutant (sRNA).

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The NRPD1 N-terminus contains a Pol IV-specific motif that is critical for genome surveillance in Arabidopsis.

Project description: Not available

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Transcriptomics of Arabidopsis thaliana nrpd1-3 and nrpd1-3 plant 2L under control or heat stress

Project description:The intent of the experiment was to infer differential heat-induced transncriptional disturbance of genes in a background with novel insertions of ONSEN/Copia78 LTR retrotransposon. For this, we performed Illumina 150 bp pair-end RNA-seq, in RdDM mutant nrpd1-3 and a line with numerous ONSEN neo-insertions (nrpd1-3 plant 2L); under control and heat stress.

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