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Cancer-associated adipocytes mRNA

ABSTRACT: The role of adipocytes in cancer microenvironment has gained focus during the recent years. However, the characteristics of the cancer-associated adipocytes (CAA) in human breast cancer tissues and the underlying regulatory mechanism are not clearly understood. We reviewed pathology specimens of breast cancer patients to understand the morphologic characteristics of CAA, and profiled the mRNA and miRNA expression of CAA by using indirect co-culture system in vitro.

ORGANISM(S): Mus musculus

PROVIDER: GSE95827 | GEO | 2017/03/09

SECONDARY ACCESSION(S): PRJNA378532

REPOSITORIES: GEO

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Cancer-associated adipocytes microRNA

Project description:The role of adipocytes in cancer microenvironment has gained focus during the recent years. However, the characteristics of the cancer-associated adipocytes (CAA) in human breast cancer tissues and the underlying regulatory mechanism are not clearly understood. We reviewed pathology specimens of breast cancer patients to understand the morphologic characteristics of CAA, and profiled the mRNA and miRNA expression of CAA by using indirect co-culture system in vitro.

2017-03-09 | GSE95828 | GEO

SAA1-dependent reprogramming of adipocytes by tumor cells is associated with triple negative breast cancer aggressiveness

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Inhibition of PPARg by BZ26, a GW9662 derivate, attenuated obesity-related breast cancer progression by inhibiting the reprogramming of mature adipocytes into to cancer associate adipocyte-like cells

Project description:Obesity has been associated with the development of 13 different types of cancers, including breast cancer. Evidence has indicated that cancer-associated adipocytes (CAAs) promote the proliferation, invasion, and metastasis of cancer. However, the mechanisms that link CAAs to the progression of obesity-related cancer are still unknown. Here, we found the mature adipocytes in the visceral fat of HFD-fed mice have a CAAs phenotype but the stromal vascular fraction (SVF) of the visceral fat has not. Importantly, we found the derivate of the potent PPARg antagonist GW9662, BZ26 inhibited the reprogramming of mature adipocytes in the visceral fat of HFD-fed mice into CAA-like cells and inhibited the proliferation and invasion of obesity-related breast cancer. Further study found that it mediated the browning of visceral, subcutaneous and perirenal fat and attenuated inflammation of adipose tissue and metabolic disorders. For the mechanism, we found that higher PPARg expression was associated with poorer overall survival of breast cancer patients, and further study showed that BZ26 bound and inhibited PPAR by acting as a new modulator. Therefore, BZ26 serves as a novel modulator of PPARg activity that is capable of inhibiting obesity-related breast cancer progression by inhibiting of CAA-like cell formation, suggesting that inhibiting the reprogramming of mature adipocytes into CAAs or CAA-like cells may be a potential therapeutic strategy for obesity-related cancer treatment.

2023-08-09 | GSE240082 | GEO

Genome‐wide analysis reveals miR‐3184‐5p and miR‐181c‐3p as a critical regulator for adipocytes‐associated breast cancer

Project description:The purpose of this study is to identify miRNA expression changes in adipocyte-associated breast cancer microenvironment by using indirect in vitro co-culture system. We performed small RNA small RNA deep sequencing in human breast cancer cells and breast cancer cells co-cultured with mature adipocytes. We identified 98 differentially expressed miRNAs in breast cancer cells co-cultured with adipocytes using small RNA deep sequencing.

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IGFBP2 secretion by mammary adipocytes limits breast cancer invasion

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Gene expression profiles of human breast cancer cell lines MCF-7, T47D and MDA-MB-231 co-cultured with 3T3-L1 adipocytes.

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2021-01-22 | GSE165273 | GEO

Effects of differentiated hMADS adipocytes on BT474 and SKBR3 breast cancer cells

2015-04-01 | GSE52660 | GEO

Effects of differentiated hMADS adipocytes on BT474 and SKBR3 breast cancer cells

Project description:Analysis of gene expression levels of HER2-positive breast cancer cells exposed to the conditioned medium from adipocytes. The hypothesis tested in the present study was that adipocytes secrete factors that induce the resistance of cancer cells to antibody-dependent cellular cytotoxicity mediated by trastuzumab. The results provide insight into the genes that may be involved in the adipocyte-induced cancer resistance to trastuzumab treatment. BT474 cells or SKBR3 cells were exposed to the conditioned medium (CM) from differentiated hMADS (#hMADS) or to the control medium for 2 h. Total RNA was extracted and analyzed. The experiment was performed in triplicate.

2015-04-01 | E-GEOD-52660 | biostudies-arrayexpress

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