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TimeLapse-seq: adding a temporal dimension to RNA sequencing through nucleoside recoding

ABSTRACT: RNA sequencing (RNA-seq) offers a snapshot of cellular RNA populations, but not temporal information about the sequenced RNA. Here we report TimeLapse-seq, which uses oxidative-nucleophilic-aromatic substitution to convert 4-thiouridine into cytidine analogs, yielding apparent U-to-C mutations that mark new transcripts upon sequencing. TimeLapse-seq is a single-molecule approach that is adaptable to many applications and reveals RNA dynamics and induced differential expression concealed in traditional RNA-seq.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE95854 | GEO | 2018/01/12

SECONDARY ACCESSION(S): PRJNA378606

REPOSITORIES: GEO

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