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Brown adipose tissue macrophages control tissue innervation and homeostatic energy expenditure

ABSTRACT: Tissue resident macrophages provide a systemic innate immune defense network and critically contribute to establishment and maintenance of tissue homeostasis. Here we used constitutive and inducible mutagenesis to delete the nuclear transcription regulator methyl-CpG binding protein 2 (MeCP2) in defined tissue macrophages. Animals lacking the Rett syndrome-associated gene in macrophages did not show signs of neurodevelopmental disorder, but surprisingly displayed altered body composition and spontaneous obesity. This phenotype involved neither hyper-phagia, primary hyper-insulinemia nor inflammation, but rather could be linked to impaired brown adipose tissue (BAT) function. Specifically, mutagenesis of a BAT-resident CX3CR1+ macrophage subpopulation compromised homeostatic, though not acute cold-induced thermogenesis. Mechanistically, steady state BAT malfunction of pre-obese mice harboring mutant macrophages was associated with decreased sympathetic innervation and lower local norepinephrine titers, resulting in reduced adipocyte expression of the thermogenic factors UCP1 and DIO2. Mutant macrophages were found to over-express PlexinA4, which might contribute to the phenotype by repulsion of Sema6A-expressing sympathetic axons. Collectively, we report a previously unappreciated homeostatic role of macrophages in the control of tissue innervation, disruption of which in BAT results in metabolic imbalance.

ORGANISM(S): Mus musculus

PROVIDER: GSE95859 | GEO | 2017/05/01

SECONDARY ACCESSION(S): PRJNA378611

REPOSITORIES: GEO

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T cells and adipocyte IL-17RC control fat innervation and thermogenesis

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2020-03-02 | PXD017424 | Pride

Mus musculus

Project description:Brown adipose tissue macrophages control tissue innervation and homeostatic energy expenditure

| PRJNA378611 | ENA

Schwann cells regulate brown adipose tissue innervation and remodeling through MEK/ERK signaling axis

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Transcription profiling of rat uterine myometrium -effect of estrogen

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2008-06-17 | E-GEOD-9117 | biostudies-arrayexpress

Expression data from rat uterine myometrium -effect of estrogen

Project description:Uterine tissue is highly responsive to estrogen, which plays a mayor role in sympathetic innervation remodeling in myometrium Microarrays were used to investigate which estrogen resposive myometrial proteins can be involved in innervation modulation Keywords: control vs. treatment, timepoints

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