Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Drosophila pole cell transcriptome in early embryogenesis (stage 4).

ABSTRACT: To screen transcripts enriched in pole cells, primordial germ cells of Drosophila, during early embryogenesis, we compared transcriptome between pole cells and whole embryos at stage 4 by a microarray analysis. We used fluorescence-activated cell sorting (FACS) to isolate pole cells from the embryos carrying the transgene, EGFP-vasa, which expresses GFP specifically and continuously in the germline throughout the life cycle. Keywords: cell type comparison

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE9679 | GEO | 2007/12/10

SECONDARY ACCESSION(S): PRJNA103571

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Drosophila PGC transcriptome

Project description:To understand transcriptomic dynamics of PGC (primordial germ cells; pole cell) of fruit fly, we used fluorescence-activated cell sorting (FACS) to isolate PGC from the embryos carrying the transgene, EGFP-vasa, which expresses GFP specifically and continuously in the germline throughout the life cycle. The PGCs collected at 11 time points during the embryogenesis were subjected to microarray analyses.

2016-12-25 | GSE83460 | GEO

Identification of the genes regulated by maternal Ovo-B in PGCs in Drosophila melanogaster.

Project description:To identify downstream genes which was regulated by maternal Ovo-B in PGCs, we compared the transcriptomes between OvoA-expressing PGCs and control PGCs by microarry analysis in Drosophila melanogaster. We used fluorescence-activated cell sorting (FACS) to isolate PGCs from the stage 16 embryos carrying the transgene, EGFP-vasa, which expresses GFP specifically and continuously in the germline throughout the life cycle.

2016-05-26 | GSE81857 | GEO

Drosophila melanogaster developmental transcriptome of whole body thorugh embryogenesis

Project description:To understand dynamics of embryonic transcriptome in a high resolution, whole bodies of embryos were collected at 12 time points during the embryogenesis and were subjected to microanalyses. These data were originally obtained aiming to compare to our other microarray data [GSE83460] of PGCs (primordial germ cells; pole cells).

2017-12-24 | GSE102307 | GEO

Transcription profiling of Xenopus pole tissue explants from Notch treated embryos vs. controls

Project description:Animal pole tissue explants were dissected from either untreated embryos or embryos injected with 1 ng of Notch Intracellular Domain RNA (NICD) at the two cell stage. Tissue explants were dissected and cultured until sibling embryos reached embryonic stage 20. Each array hybridization represents a biological replicate containing approximately 100 tissue explants. Expression profiles were determined using Affymetrix Xenopus Genechip 1 arrays. Experiment Overall Design: 4 untreated pooled explants, 2 NICD treated explants

2008-06-13 | E-GEOD-5564 | biostudies-arrayexpress

Epigenome Analysis of Post-Mortem Human Temporal Pole Brain Tissue

Project description:Epigenome Analysis of Post-Mortem Human Temporal Pole Brain Tissue For more information, please refer to DOI: 10.3233/JAD-141989 Temporal Pole regions from 24 age-matched causcasian males: 8 samples which died of normal causes, 8 samples with Alzheimer's disease (stage 3/4) and 8 samples with dementia with lewy bodies

2015-03-31 | E-GEOD-67419 | biostudies-arrayexpress

Epigenome Analysis of Post-Mortem Human Temporal Pole Brain Tissue

Project description:Epigenome Analysis of Post-Mortem Human Temporal Pole Brain Tissue For more information, please refer to DOI: 10.3233/JAD-141989

2015-03-31 | GSE67419 | GEO

Avelumab for MSI-H or POLE Mutated Metastatic Colorectal Cancer

Project description:The POLE mutations represent high somatic mutation loads in patients with colorectal cancer, especially in those with MMR proficient or MSS, therefore, tumors harbouring POLE mutations might be susceptible to immune checkpoint blockade. Based on these reasons, Investigator planned a phase II study of avelumab monotherapy in patients with previously treated, metastatic, MMR deficient (MSI-H) or POLE mutated colorectal cancer.

| 2244190 | ecrin-mdr-crc

Durvalumab for MSI-H or POLE Mutated Metastatic Colorectal Cancer

Project description:The POLE mutations represent high somatic mutation loads in patients with colorectal cancer, especially in those with MMR proficient or MSS, therefore, tumors harbouring POLE mutations might be susceptible to immune checkpoint blockade. Based on these reasons, the investigators planned a phase II study of durvalumab monotherapy in patients with previously treated, metastatic, MMR deficient (MSI-H) or POLE mutated colorectal cancer.

| 2266020 | ecrin-mdr-crc

Maternally inherited piRNAs silence transposons during Drosophila embryogenesis

Project description:The piRNA pathway controls transposon expression in animal germ cells, thereby ensuring genome stability over generations. piRNAs are maternally deposited and required for proper transposon silencing in adult offspring. However, a long-standing question in the field is the precise function of maternally deposited piRNAs and its associated factors during embryogenesis. Here, we probe the spatio-temporal expression patterns of several piRNA pathway components during early stages of development. Amongst those, factors required for transcriptional gene silencing (TGS) showed ubiquitous abundance in somatic and pole cells throughout the first half of embryogenesis. We further analysed the transcriptomes of various embryo stages and correlated these with the presence of selected chromatin marks. We found that a number of transposon families show bursts of transcription during early embryonic stages. Transposons heavily targeted by maternally deposited piRNAs accumulated repressive chromatin marks following their spike in expression. Furthermore, depletion of maternally deposited Piwi protein in early embryos resulted in increased expression of transposons targeted by inherited piRNAs and was accompanied by a strong loss of repressive chromatin marks at coding sequences. Overall, our data suggests a pivotal role for the piRNA pathway in transposon defence during Drosophila embryogenesis in somatic cells.

2021-07-26 | PXD022409 | Pride

RNA sequencing of pole-containing anterior tip regions in planarian Schmidtea mediterranea

Project description:RNA sequencing identified new anterior-pole enriched genes encoding transcription factors, a secreted protein, and a transmembrane protein, including NR4A, a nuclear receptor gene that is important in patterning the planarian head and tail.

2019-05-25 | GSE121045 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data