Transcriptomics,Genomics

Dataset Information

145

Single-cell RNAseq analysis of the empty and i8TF cell lines after 3 days of BL-CFC culture


ABSTRACT: The aim of the experiment was to assess the cell heterogeneity after the doxycycline treatment and the subsequent induction of the 8 transcription factors in the BL-CFC culture. Overall design: A2lox.empty and A2lox.i8TFs cell lines were used for this experiment. A2lox.empty is used a negative control while the doxycycline is inducing the expression of Erg, Fli1, Tal1, Lyl1, Lmo2, Runx1, Cbfb and Gata2 in the A2lox.i8TFs cell line. Cells were cultured in BL-CFC culture for 1 day before doxycycline treatment and were cultured for an additional 48hours with (i8TFs_plus and E_plus) or without doxycycline (i8TFs_minus and E_minus). As additional controls of the single cell RNA seq experiment, bulk A2lox.i8TFs cells were collected at the same time point from the condition without doxycycline (Pos_Ctrl), also buffer only was used as negative control (Neg_Ctrl) and a human cell line as other control (K562).

INSTRUMENT(S): Illumina NextSeq 500 (Homo sapiens)

SUBMITTER: Christophe Lancrin  

PROVIDER: GSE96982 | GEO | 2018-03-20

REPOSITORIES: GEO

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Publications


Recent advances in single-cell transcriptomics techniques have opened the door to the study of gene regulatory networks (GRNs) at the single-cell level. Here, we studied the GRNs controlling the emergence of hematopoietic stem and progenitor cells from mouse embryonic endothelium using a combination of single-cell transcriptome assays. We found that a heptad of transcription factors (Runx1, Gata2, Tal1, Fli1, Lyl1, Erg and Lmo2) is specifically co-expressed in an intermediate population expressi  ...[more]

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