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Effects of arsenic and Pseudomonas aeruginosa infection on gene expression in human airway epithelial cells

ABSTRACT: Purpose: To determine effects of arsenic on gene expression in polarized primary human bronchial epithelial (HBE) cells and impact on transcriptional response to Pseudomonas aeruginosa infection Methods: mRNA profiles of HBE cells from 6 donors exposed to 0, 5, 10 or 50 ug/L total arsenic +/- Pseudomonas aeruginosa (48 samples) were generated using Illumina sequencing, aligned in CLC Genomics workbench and analyzed for DE in EdgeR Findings: 20-30 million reads were mapped per sample and transcripts were identifed that were significantly differentially expressed in response to arsenic and Pseudomonas aeruginosa

ORGANISM(S): Homo sapiens

PROVIDER: GSE97036 | GEO | 2017/06/26

SECONDARY ACCESSION(S): PRJNA380496

REPOSITORIES: GEO

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