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Expression data from human glioma cells U251 or LN-319 with stable expression of GFP control or sh-Circ-SHPRH

ABSTRACT: The cDNA array of U251 or LN-319 cells stably infected with sh-Circ-SHPRH or control lenti-virus.

ORGANISM(S): Homo sapiens

PROVIDER: GSE97185 | GEO | 2017/03/30

SECONDARY ACCESSION(S): PRJNA380923

REPOSITORIES: GEO

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Homo sapiens

Project description:Expression data from human glioma cells U251 or LN-319 with stable expression of GFP control or sh-Circ-SHPRH

| PRJNA380923 | ENA

circ-ZNF609 regulates G1-S progression in Rhabdomyosarcoma

Project description:Circular RNAs (circRNAs) represent a class of covalently closed RNAs, derived from a non-canonical splicing event, ubiquitously expressed among Eukaryotes and conserved among different species. We identified a circRNA (circ-ZNF609) involved in the regulation of human primary myoblast proliferation. Upon its depletion, the percentage of proliferating myoblasts was highly reduced. To deepen our knowledge about circ-ZNF609 role in cell cycle regulation, we studied its expression and function in Rhabdomyosarcoma (RMS), a pediatric skeletal muscle malignancy. We found that circ-ZNF609 is up-regulated in biopsies from both the two major RMS subtypes, the alveolar (ARMS) and the embryonal (ERMS), and we discovered that its knock-down blocks proliferation of an ERMS-derived cell line, while it has no effect on ARMS-derived cells. To understand the mechanism through which circ-ZNF609 affects cell proliferation we compared the different effects of circ-ZNF609 depletion in ERMS and ARMS and we identified genes and pathways on which the circRNA acts.

2018-12-14 | GSE117609 | GEO

LN immunoinfiltrate analysis

Project description:LN immunoinfiltrate analysis

| PRJNA634254 | ENA

Circ-Phkb promote LPS-induced acute lung injury via the TLR4/MyD88/NF-κB /CCL2 axis

Project description:Our results showed that both circRNA and mRNA profiles are different from those of the sham group. A significant upregulation of circ-Phkb was observed in NR8383 cells and rats exposed to LPS. When circ-Phkb expression was reduced in NR8383 cells, apoptosis and inflammation were greatly reduced, and cell proliferation was increased, while overexpression of circ-Phkb have the opposite effect. In terms of mechanism, circ-Phkb suppression inhibits CCL2 expression via TLR4/MyD88/NF-κB pathway.

2024-02-07 | GSE226036 | GEO

Sinorhizobium meliloti strain:319

Project description:Sinorhizobium meliloti 319 Resequencing

| PRJNA501524 | ENA

U251 LC-MSMS - U251 cells were treated with Vinblastine or Taxol and phosphotyrosine signaling was measured.

Project description:U251 cells were treated with Vinblastine or Taxol and phosphotyrosine signaling was measured.

2018-11-30 | PXD008939 | Pride

Corynebacterium sp. 319

Project description:Corynebacterium sp. 319 Genome sequencing and assembly

| PRJNA574707 | ENA

Mechanism of Nootkatone in U251

Project description:Nootkatone can induce the changes of gene expression in U251 cells

2023-07-02 | GSE235973 | GEO

PRMT3 drives glioblastoma progression by enhancing HIF1A and glycolytic metabolism

Project description:To investigate the potential mechanisms underlying PRMT3 regulation of GBM growth, we performed RNA-seq transcriptomic profiling of U251 GBM cells treated with lenti-ShCtrl and shPRMT3.

2022-11-08 | GSE200902 | GEO

Study on the mechanism of circ-PDE5A inhibiting the progression of esophageal squamous cell carcinoma

Project description:Objective: Esophageal squamous carcinoma (ESCC) is one of the most common gastrointestinal tumors, and the PI3K/AKT signaling pathway plays a key role in the development of ESCC. circRNAs have been reported to be involved in the regulation of PI3K/AKT signaling, but the underlying mechanisms are unclear. This study aimed to identify protein-coding circRNAs and investigate their function in ESCC. Design: Differential expression of circRNAs between ESCC tissues and adjacent normal tissues was identified using circRNA microarray analysis. A novel protein encoded by circ-PDE5A was identified by LC-MS/MS. Molecular biological methods were used to explore the biological functions and regulatory mechanisms of circ-PDE5A and its encoded PDE5A-500aa novel protein in ESCC. Construction of a nanoplatform for the coupling of circRNAs to investigate the therapeutic translation value of circ-PDE5A. Results: We found that circ-PDE5A expression was downregulated in ESCC cells and tissues, and its low expression was associated with later clinicopathological staging and poorer prognosis. Functionally, circ-PDE5A inhibited ESCC proliferation and metastasis in vitro and in vivo by encoding the novel PDE5A-500aa protein, which was identified as a key player in regulating PI3K/AKT signaling activation in ESCC. Mechanistically, the novel PDE5A-500aa protein binds directly to PIK3IP1 and promotes USP14-mediated deubiquitination of the k48-linked polyubiquitin chain at residue K198 of PIK3IP1, thereby attenuating PI3K/AKT pathway in ESCC. In addition, the circ-PDE5A plasmid-coupled reduction-responsive nanoplatform successfully inhibited ESCC growth and metastasis. Conclusions: circ-PDE5A represents an epigenetic mechanism regulating PI3K/ATK signaling and serves as a novel and promising therapeutic target and prognostic marker for ESCC.

2024-01-15 | GSE250413 | GEO

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