Transcriptomics,Genomics

Dataset Information

154

De novo reconstruction of human adipose reveals conserved lncRNAs as regulators of brown adipogenesis


ABSTRACT: Obesity has emerged as a formidable health crisis due to its association with metabolic risk factors such as diabetes, dyslipidaemia and hypertension. Recent work has demonstrated the multifaceted roles of lncRNAs in regulating mouse adipose development, but its implication in human adipocytes remain largely unknown at least partially due to the lack of a comprehensive lncRNA catalog, particularly those specifically expressed in brown adipose tissue (BAT). In this study, we performed deep RNA-seq on adult subcutaneous, omental and fetal brown adipose tissues to de novo construct a catalog of 3,149 adipose active lncRNAs of which 1,351 are specifically detected in BAT. We further identified 318 lncRNAs conserved between human and mouse which, compared with non-conserved ones, are more broadly expressed in multiple cell types. One of these, lnc-dPRDM16, is transcribed divergently from Prdm16, tightly correlated with Prdm16 (R ≥ 0.7) in both mouse and human, and co-expressed (R ≥ 0.7) with protein-coding genes enriched in lipid and fatty acid catabolic processes. Loss of function of lnc-dPRDM16 led to a down-regulation of Prdm16 and an obvious reduction of adipogenesis in brown adipocyte culture. Together, our work has provided a comprehensive human adipose catalog built from diverse fat types, which when applied to our roadmap, identifies lnc-dPRDM16 as a promising modulator of adipose development for future clinical research. Overall design: Transcriptome profiling of BAT, OME and SUB samples

INSTRUMENT(S): Illumina HiSeq 2000 (Homo sapiens)

SUBMITTER: Lei Sun  

PROVIDER: GSE97205 | GEO | 2018-04-16

REPOSITORIES: GEO

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De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis.

Ding Chunming C   Lim Yen Ching YC   Chia Sook Yoong SY   Walet Arcinas Camille Esther ACE   Xu Shaohai S   Lo Kinyui Alice KA   Zhao Yanling Y   Zhu Dewen D   Shan Zhihui Z   Chen Qingfeng Q   Leow Melvin Khee-Shing MK   Xu Dan D   Sun Lei L  

Nature communications 20180406 1


Obesity has emerged as an alarming health crisis due to its association with metabolic risk factors such as diabetes, dyslipidemia, and hypertension. Recent work has demonstrated the multifaceted roles of lncRNAs in regulating mouse adipose development, but their implication in human adipocytes remains largely unknown. Here we present a catalog of 3149 adipose active lncRNAs, of which 909 are specifically detected in brown adipose tissue (BAT) by performing deep RNA-seq on adult subcutaneous, om  ...[more]

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