Genomics

Dataset Information

38

Porcine oocytes maturation in vitro


ABSTRACT: The proper mammalian oocytes maturation is recognized as reaching MII stage and accumulation of mRNA and proteins in cell cytoplasm following fertilization. The proper course of folliculogenesis and oogenesis is orchestrated with morphogenesis significantly influencing further zygote formation and embryos growth. This study was aimed to determinate new transcriptomic markers of porcine oocytes morphogenesis associated with cell maturation capacity. We used microarrays to detail the global programme of gene expression underlying changes before and after in vitro maturation of oocytes in sus scrofa Overall design: Pig oocytes were aspired from porcine ovaries, selected to two groups by BCB test, subjected to in vitro maturation, RNA extraction, reverse transcription, and hybridization on Affymetrix microarrays. Only oocytes of grade I with homogeneous ooplasm were considered for the following steps of the experiment, resulting 300 grade I oocytes (3 x n=50 before in vitro maturation, 3 x n=50 after In vitro maturation).

INSTRUMENT(S): [PorGene-1_1-st] Porcine Gene 1.1 ST Array [transcript (gene) version]

SUBMITTER: Bartosz Kempisty  

PROVIDER: GSE97246 | GEO | 2017-03-31

SECONDARY ACCESSION(S): PRJNA381067

REPOSITORIES: GEO

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Publications

"Cell Migration" Is the Ontology Group Differentially Expressed in Porcine Oocytes Before and After In Vitro Maturation: A Microarray Approach.

Kranc Wiesława W   Budna Joanna J   Chachuła Adrian A   Borys Sylwia S   Bryja Artur A   Rybska Marta M   Ciesiółka Sylwia S   Sumelka Ewa E   Jeseta Michał M   Brüssow Klaus P KP   Bukowska Dorota D   Antosik Paweł P   Bruska Małgorzata M   Nowicki Michał M   Zabel Maciej M   Kempisty Bartosz B  

DNA and cell biology 20170216 4


Maturation of cumulus-oocyte complexes (COCs) is crucial for further successful monospermic fertilization, embryo growth, and implantation. All these events are accompanied by proliferation and differentiation of cumulus cells. The migration of COCs to the oviduct after ovulation and the interaction between female gametes and/or embryos with maternal tissues are still poorly recognized on the molecular level. This study was aimed to first demonstrate the mRNA expression profile of cell migration  ...[more]

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