Transcriptomics,Genomics

Dataset Information

43

MiRNA data in human placentas from normal pregnancies and from pregnancies complicated by SGA and IUGR [miRNA-4]


ABSTRACT: Placental insufficiency leading to intrauterine growth restriction demonstrates perturbed gene expression affecting placental angiogenesis and nutrient transfer from mother to fetus. To understand the post-transcriptional mechanisms underlying such placental gene expression changes, our objective was to identify key non-coding microRNAs that express biological function. To this end, we undertook microarray targeting microRNAs in placentas of appropriate (AGA, n=3) versus small for gestational age (SGA, n=3) weight infants and observed upregulation of 97 miRs in SGA versus AGA. In a larger cohort of samples, we validated by qRT-PCR, differential expression of three specific microRNAs that target genes mediating angiogenesis and nutrient transfer. This dataset was used to verify initial studies. We performed a second microarray on a small cohort of samples in order to validate differential expression in pathways involved with angiogenesis and nutrient transfer, essential processes for placental function that when perturbed, may result in intrauterine growth restriction. Overall design: Human placental samples were obtained immediately post-parturient. There were seven samples from the AGA group (Bw>10% and <90%) and six samples from the SGA/IUGR (Birthweight <10%) group. All placental samples were from term, singleton pregnancies.

INSTRUMENT(S): [miRNA-4] Affymetrix Multispecies miRNA-4 Array [ProbeSet ID version]

SUBMITTER: Alison Chu  

PROVIDER: GSE97294 | GEO | 2017-04-01

SECONDARY ACCESSION(S): PRJNA381214

REPOSITORIES: GEO

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Publications

Differential microRNA expression in human placentas of term intra-uterine growth restriction that regulates target genes mediating angiogenesis and amino acid transport.

Thamotharan Shanthie S   Chu Alison A   Kempf Katie K   Janzen Carla C   Grogan Tristan T   Elashoff David A DA   Devaskar Sherin U SU  

PloS one 20170502 5


Placental insufficiency leading to intrauterine growth restriction (IUGR) demonstrates perturbed gene expression affecting placental angiogenesis and nutrient transfer from mother to fetus. To understand the post-transcriptional mechanisms underlying such placental gene expression changes, our objective was to identify key non-coding microRNAs that express biological function. To this end, we initially undertook microarrays targeting microRNAs in a small sub-set of placentas of appropriate (AGA)  ...[more]

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