Dataset Information

Study on the mechanism of Shcisandrol A in immune function modulation in mice based on the mRNA expression profile chip

ABSTRACT: At present, with the accelerating pace of life, increase in work pressure, lack of exercise, irregularity of diet and rest, and problems of environmental pollution, people with immunodeficiency are gradually increasing in the modern population. Some Chinese herbal medicines can improve the immunity of the human body, and have less and mild side or toxic effects, so that the study on this kind of traditional Chinese medicines and its mechanism has become an urgent problem to be solved. In recent years, studies have shown that Shcisandrol A (Sch A) can regulate immune function and inhibit the inflammation of nervous system. In this study, mRNA expression profile chip was used to screen the differentially expressed genes related to the effect of Sch A on immunodepressed mice induced by cyclophosphamide (Cy), and the differentially expressed gene-telated pathways were analyzed by gene ontology function cluster analysis, and finally qPCR was applied to verify the 5 genes that might be related to the regulation of Sch A on the mice’s immune functions, to provide a theoretical basis for screening the drug targets on which Sch A could act to regulate the mice’s immune functions.

ORGANISM(S): Mus musculus

PROVIDER: GSE97316 | GEO | 2020/04/03

REPOSITORIES: GEO

ACCESS DATA

Similar Datasets

Project description:The immune system is our body’s defense mechanism, which helps us to resist foreign pathogens and cancer cells in the body. However, if the immune system is too weak, too strong, or unbalanced, it will be susceptible to get bacterial and viral infections, unable to fight cancer, or cause allergies, autoimmune and rheumatic diseases. At present, there is no good western medicine to strengthen immunity when the body is insufficient immunity. However, strong immunity causes autoimmune diseases and need to be treated by steroids and immunosuppressive agents. These drugs have limited efficacy and often have many side effects. In the clinical practice of traditional Chinese medicine, tonify qi, supplement blood and nourish yin are often used to enhance immunity. However, there is no clinical test to demonstrate the modulation of immune response after traditional Chinese medicine treatment. In our preliminary study, the investigators identified that the basic immune response of cancer patients is weaker than healthy people by testing the level of IFN-r (promoting T cell activity, anti-tumor, anti-viral), TNF-a (promoting B cell proliferation, producing antibodies, anti-tumor, anti-viral) and IL-2 (regulate the proliferation and differentiation). Besides, some Chinese herbal extracts can significantly stimulate the immune response higher than the general average basic immunity of cancer patients. Further, we will use the stimulatory Chinese medicine extract to administer to cancer patients and examine the immune response of cancer patients after taking them. Therefore, this test can provide actual data on the basic immunity of healthy people or cancer patients and the modulation of immune response by traditional Chinese medicine treatment. This analysis platform provides patients to select the medicines that can enhance the immune response for individuals and analyze the changes of the immune response after conditioning with the candidate Chinese medicines. Therefore, this test could be applied clinically to validate the immunological regulation of Chinese herbal medicine as well as analyze the immunological modulation against different cancer by various Chinese herbal medicine.

Dataset Information

Study on the mechanism of Shcisandrol A in immune function modulation in mice based on the mRNA expression profile chip

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure