Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome Comparison of phoU Homologies Genes Deletion strain and the Parent Strain in log-phase (6 h).

ABSTRACT: Purpose: To compare the transcriptome changes in phoU1(serp0956) or phoU2(serp0316)deletion strain with the parent strain SE1457 in log-phase (6 h) Methods: Total RNA was isolated and sequenced from the phoU1 deletion strain, phoU2 deletion strain and the parent strain SE1457 in log-phase (6 h) in triplicate using an illumina high-seq 2500. Raw data was analyzed using TopHat. Genes were considered changed which performed fold-change>=1.5 and FDR<=0.05. Changed genes were then filtered to reveal the downstream targets of phoU1 or phoU2 in log-phase (6 h). Results: The expression of 23 genes were significantly decreased and 69 genes was significantly increased in expression level due to the loss of phoU1 expression.The expression of 474 genes were significantly decreased and 471 genes were significantly increased in expression level due to the loss of phoU2 expression.

ORGANISM(S): Staphylococcus epidermidis

PROVIDER: GSE97400 | GEO | 2017/10/03

SECONDARY ACCESSION(S): PRJNA381636

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Transcriptome Comparison of phoU Homologies: Genes Deletion strain and the Parent Strain in stationary phase (10 h)

Project description:Purpose: To compare the transcriptome changes in phoU1(serp0956) or phoU2(serp0316)deletion strain with the parent strain SE1457 in stationary phase (10 h). Methods: Total RNA was isolated and sequenced from the phoU1 deletion strain, phoU2 deletion strain and the parent strain SE1457 in stationary phase (10 h) in triplicate using an illumina high-seq 2500. Raw data was analyzed using TopHat. Genes were considered changed which performed fold-change>=1.5 and FDR<=0.05. Changed genes were then filtered to reveal the downstream targets of phoU1 or phoU2. Results: The expression of 2 genes were significantly decreased and no genes was significantly increased in expression level due to the loss of phoU1 expression.The expression of 279 genes were significantly decreased and 716 genes were significantly increased in expression level due to the loss of phoU2 expression.

2017-10-03 | GSE97656 | GEO

Expression profile of a parent and M-bM-^HM-^FlstC strain in regular and high salt conditions

Project description:Microarray analysis of the expression profiles of a parent and its derived M-bM-^HM-^FlstC mutant in normal and high salt conditions. The analysis was performed to determine which genes were altered in the mutant when grown in high salt conditions relative to the parent strain. Cultures were performed in triplicate and collected during mid-log phase growth in normal and high salt conditions. RNA was extracted for Affymetrix microarray analysis and compared to identify genes significantly altered in their expression using R and Bioconductor.

2014-04-16 | E-GEOD-56828 | biostudies-arrayexpress

Halobacterium NRC-1 ura3 parent strain and VNG1451C deletion mutant in response to growth in rich medium

Project description:Gene regulatory networks play an important role in coordinating biochemical fluxes through diverse metabolic pathways. The modulation of enzyme levels enables efficient utilization of limited resources as organisms dynamically acclimate to nutritional fluctuations in their environment. Here we have identified and characterized a novel nutrient-responsive transcription factor from the halophilic archaea, VNG1451C. In this experiment we used whole-genome microarray analysis in the VNG1451C deletion mutant vs. H. salinarum NRC-1 ura3 parent strain in rich medium during growth to show that the expression of many metabolic genes is perturbed in the VNG1451C deletion mutant. Halobacterium salinarum NRC-1 (ATCC700922) ura3 parent and VNG1451C strains were grown in complex medium (CM; 250g/L NaCl, 20g/L MgSO4.7H2O, 3g/L sodium citrate, 2g/L KCl, 10g/L peptone) at 37ÂºC under full-spectrum white light. Biological replicate samples were removed throughout the growth curve at early log, mid log, late log, and stationary phase to measure genome-wide transcription.

2010-05-06 | E-GEOD-13531 | biostudies-arrayexpress

Staphylococcus epidermidis

Project description:Transcriptome Comparison of phoU Homologies Genes Deletion strain and the Parent Strain in log-phase (6 h).

| PRJNA381636 | ENA

Histone dosage and gene expression changes in Halobacterium salinarum

Project description:We set out to determine a) if histone in Halobacterium salinarum regulates transcription and b) whether the magnitude and extent of these changes matches those observed in organisms which use histone protein as their primary DNA packaging agent. To this end, gene expression data for a histone knock-out (?ura3?hpyA) strain versus parent (?ura3) were collected. The histone deletion mutant and parent strain, at log and stationary phase, were compared to the common reference strain NRC-1 (log). There are three biological replicates each, plus dye-flips, for a total of 24 arrays

2015-08-31 | E-GEOD-54599 | biostudies-arrayexpress

mRNA expression in TTHA0118 deletion mutant of Thermus thermophilus HB8 strain grown on minimum essential medium

Project description:We compared the expression profile of TTHA0118 deletion mutant strain of Thermus thermophils HB8 with that of wild-type. The mutant strain grown in minimum essential medium (CS medium) exhibited growth retardation. In early log phase, 29 genes were suppressed and 25 genes were activated for the mutant strain. The suppressed genes included genes of electron transport, and cell growth and division. The activated genes included genes of stress response. We suggested that these transcriptional alterations in the mutant cell were induced by the pAp accumulation and mononucleotides starvation. Keywords: cell type comparison Keywords: cell type comparison

2008-12-31 | GSE12590 | GEO

Comparison of the wild-type strain H99W with the hva1∆, hva1∆+HVA1 and a Galleria-passaged strain

Project description:Changes in gene expression of the serotype A wild-type strain H99W with the hva1∆ (8), hva1∆+HVA1 (8-15) or a Galleria-passaged (P15) strain were examined during log-phase growth at 37 ºC.

2016-08-11 | GSE59582 | GEO

Growth phase dependent gene expression of Salmonella Typhimurium SL1344 parental strain and isogenic ∆relA, ∆spoT ppGpp null strain

Project description:Transcriptional profiling of Salmonella Typhimurium SL1344 parental starin and isogenic ∆relA, ∆spoT ppGpp null strain grown in LB medium with RNA samples talken at AD600=1.0 (mid log, ML), 2.3 (early stationary phase, ESP), 3.0 (mid stationary phase MSP) and 3.6 (Late stationary phase (LSP)

2013-07-03 | GSE48478 | GEO

Gene expression profiling of M tuberculosis BCG strain during extended stationary and resuscitation phase of growth

Project description:Objective of the study is to find out the differentially regulated genes of Mycobacterium BCG strain in extended stationary phase comparison to log phase growth and resuscitation phase. Mycobacterium BCG culture was grown in Sauton medium at 37o C without shaking. Cells at A600 0.6-0.8 were harvested as log phase culture. The cells harvested after 5 months incubation at 37oC without shaking became non culturable and were harvested as extended stationary phase cells. The extended stationary phase cells were treated with resuscitation promoting factor (Rpf) from Micrococcus luteus and harvested after 8 days. Cells of this stage were treated as resuscitation phase cells. Gene expression profiling was carried out using Agilent microarray platform. Keywords: Extended stationary phase, Log phase growth and Resuscitation phase.

2009-07-01 | GSE15642 | GEO

Transcription profiling of Pseudomonas aeruginosa parental strain PAO1 and PAOQ_psrA::Tn5 knock out mutant

Project description:5 Biological repeats of bacterial strain PAO1(parent, control) were compared to 5 biological repeats of PAO1_psrA::Tn5 knockout mutant, both strains were grown in BM2-glucose media containing 2mM MgSO4. Cells were grown to mid-log phase, OD600 0.50 Cells were immediately harvested and RNA was extracted

2008-07-04 | E-FPMI-14 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data