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Differentially expressed genes from RNA-Seq and functional enrichment results are affected by the choice of single-end versus paired-end reads and stranded versus non-stranded protocols

ABSTRACT: We undertook four mammalian transcriptomics experiments to compare the effect of read mapping, feature counting and differential expression analysis using single-end (SE) and paired-end (PE) protocols. For three of these experiments we also compared a non-stranded (NS) and a strand-specific approach to mapping the paired-end data.

OTHER RELATED OMICS DATASETS IN: PRJNA381932

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE97471 | GEO | 2017/06/09

SECONDARY ACCESSION(S): PRJNA381932

REPOSITORIES: GEO

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