CD27 marks embryonic hematopoietic stem cells

ABSTRACT: Cell surface markers of adult hematopoietic stem cells (HSCs) are well established, but fewer markers are available for embryonic HSCs and their immediate precursors (pre-HSCs) in the major arteries. We performed RNA-Seq on a population of cells from the major arteries of embryonic day 11.5 mouse embryos enriched for lymphoid progenitors, pre-HSCs, and HSCs. We determined that Tnfrsf7, encoding CD27, a member of the tumor necrosis factor receptor superfamily, is upregulated in this population. CD27 is found on ~5% of hematopoietic cluster cells in the major arteries, and enriches for progenitors with lymphoid potential, embryonic HSCs, and Type II pre-HSCs (CD144+CD45+), and fetal liver HSCs. It does not, however, enrich for yolk sac derived erythro-myeloid progenitors. Collectively, those data show that CD27 is a new cell surface marker of embryonic HSCs and Type II pre-HSCs.

ORGANISM(S): Mus musculus

PROVIDER: GSE97509 | GEO | 2017/09/06

SECONDARY ACCESSION(S): PRJNA382138

REPOSITORIES: GEO