Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

DNA methylation analysis of bull semen according to peripubertal age (12vs16 mo)

ABSTRACT: The DNA methylation pattern in spermatozoa of bulls at different age was investigated. Spermatozoa collected from 4 different bulls at 12 and 16 month each. Genome-wide DNA methylation analysis was performed by micro array using EDMA platform.

ORGANISM(S): Bos taurus

PROVIDER: GSE97521 | GEO | 2017/04/08

SECONDARY ACCESSION(S): PRJNA382202

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

DNA methylation analysis of bull semen according to peripubertal age (10vs16 mo)

Project description:The DNA methylation pattern in spermatozoa of bulls at different age was investigated. Spermatozoa collected from 4 different bulls at 10 and 16 month each. Genome-wide DNA methylation analysis was performed by micro array using EDMA platform.

2017-04-08 | GSE97520 | GEO

DNA methylation analysis of bull semen according to peripubertal age (10 months vs. 16 months)

Project description:The DNA methylation pattern in spermatozoa of bulls at different age was investigated. Spermatozoa collected from 4 different bulls at 10, 12 and 16 month each. Genome-wide DNA methylation analysis was performed by micro array using EDMA platform. three condition experiment, 4 bulls, all collected at 10, 12 and 16 month

2016-02-24 | E-GEOD-78231 | biostudies-arrayexpress

DNA methylation analysis of bull semen according to peripubertal age (12 months vs. 16 months)

2016-02-24 | E-GEOD-78232 | biostudies-arrayexpress

Transcriptomic analysis of blastocyst according to peripubertal age of bull (12 vs 16 months)

Project description:The transcriptome pattern in blastocyst produced by bulls at different age was investigated. Embryos were in vitro fertilized from 4 different bulls at 12 and 16 month each with matched adult cows. Genome-wide DNA methylation analysis was performed by micro array using EDMA platform.

2020-04-02 | GSE128356 | GEO

Transcriptomic analysis of blastocyst according to peripubertal age of bull (10 vs 16 months)

Project description:The transcriptome pattern in blastocyst produced by bulls at different age was investigated. Embryos were in vitro fertilized from 4 different bulls at 10 and 16 month each with matched adult cows. Genome-wide DNA methylation analysis was performed by micro array using EDMA platform.

2020-04-02 | GSE128355 | GEO

DNA methylation analysis of blastocyst according to peripubertal age of bull (12 vs 16 months)

Project description:The DNA methylation pattern in blastocyst produced by bulls at different age was investigated. Embryos were in vitro fertilized from 4 different bulls at 12 and 16 month each with matched adult cows. Genome-wide DNA methylation analysis was performed by micro array using EDMA platform.

2020-04-02 | GSE128354 | GEO

DNA methylation analysis of blastocyst according to peripubertal age of bull (10 vs 16 months)

Project description:The DNA methylation pattern in blastocyst produced by bulls at different age was investigated. Embryos were in vitro fertilized from 4 different bulls at 10 and 16 month each with matched adult cows. Genome-wide DNA methylation analysis was performed by micro array using EDMA platform.

2020-04-02 | GSE128353 | GEO

Metabolomic fingerprinting of bull spermatozoa for identification of fertility signature metabolites

Project description:The objective of the study was to identify the fertility-associated metabolites in bovine spermatozoa using liquid chromatography-mass spectrometry (LC-MS). Six Holstein Friesian crossbred bulls (three high-fertile and three low-fertile bulls) were the experimental animals. Sperm proteins were isolated and protein-normalized samples were processed for metabolite extraction and subjected to LC-MS/MS analysis. Mass spectrometry data were processed using iMETQ software and metabolites were identified using Human Metabolome DataBase while, Metaboanalyst 4.0 tool was used for statistical and pathway analysis. A total of 3,704 metabolites belonging to various chemical classes were identified in bull spermatozoa. After sorting out exogenous metabolites, 56 metabolites were observed common to both the groups while 44 and 35 metabolites were found unique to high- and low-fertile spermatozoa, respectively. Among the common metabolites, concentrations of 19 metabolites were higher in high-fertile compared to low-fertile spermatozoa (fold change > 1.00). Spermatozoa metabolites with variable importance in projections score of more than 1.5 included hypotaurine, d-cysteine, selenocystine. In addition, metabolites such as spermine and l-cysteine were identified exclusively in high-fertile spermatozoa. Collectively, the present study established the metabolic profile of bovine spermatozoa and identified the metabolomic differences between spermatozoa from high- and low-fertile bulls. Among the sperm metabolites, hypotaurine, selenocysteine, l-malic acid, d-cysteine, and chondroitin 4-sulfate hold the potential to be recognized as fertility-associated metabolites.

2021-12-07 | MTBLS1071 | MetaboLights

Effect of cryopreservation on bull sperm proteins

Project description:In the present study, we evaluated the alteration of protein profile of spermatozoa during the different stages of cryopreservation i.e., freshly ejaculated sperm, equilibrated sperm, and cryopreserved sperm in crossbred bulls (Bos taurus * Bos indicus). It was found that the equilibration step of cryopreservation itself caused major changes in the proteome of spermatozoa. Therefore, cryopreservation protocols should be tailored in such a way that it minimize sperm proteome alterations.

2022-02-25 | PXD031895 | JPOST Repository

Bull sperm SWATH-MS-based proteomics reveals link between high fertility and energy production, motility structures, and sperm-oocyte interaction. Quantitation

Project description:Prediction of male or semen fertility potential remains a persistent challenge that has yet to be fully resolved. This work analyzed several in vitro parameters and proteome of spermatozoa in bulls cataloged as high (HF; n=5) and low field (LF; n=5) fertility after more than a thousand artificial inseminations. Sperm motility was evaluated by Computer-Assisted Sperm Analysis. Sperm viability, mitochondrial membrane potential (MMP), and reactive oxygen species (mROS) of spermatozoa were assessed by flow cytometry. Proteome was evaluated by SWATH-MS procedure. Spermatozoa of HF bulls showed significantly higher total motility than the LF group (41.4% vs. 29.7%). Rates of healthy sperm (live, high MMP, and low mROS) for HF and LF bull groups were 49% and 43%, respectively (p > 0.05). Spermatozoa of HF bulls showed higher presence of differentially abundant proteins (DAPs) related to both energy production (COX7C), mainly OXPHOS pathway, and to the development of structures linked with the motility process (TPPP2, SSMEM1 and SPAG16). Furthermore, we observed that EQTN, together with other DAPs related to the interaction with the oocyte, were overrepresented in HF bull spermatozoa. The biological processes related to protein processing, catabolism, and protein folding were found to be overrepresented in LF bull sperm in which the HSP90AA1 chaperone was identified as the most DAP

2023-10-18 | PXD042286 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data