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Gene Expression-Based High Throughput Screening: APL Treatment with Candidate Compounds

ABSTRACT: We developed a general approach to small molecule library screening called GE-HTS (Gene Expression-Based High Throughput Screening) in which a gene expression signature is used as a surrogate for cellular states and applied it to the identification of compounds inducing the differentiation of acute myeloid leukemia cells. In screening 1,739 compounds, we identified 8 that reliably induced the differentiation signature, and furthermore yielded functional evidence of bona fide differentiation. We tested several of these in duplicate replicates in blasts from a patient with APL. Also included in this data set are a collection of 6 primary patient AML cells, 3 normal neutrophils samples, and 3 normal monocyte samples. This data was used to evaluate whole genome effects of the compounds on APL cells in relation to AML versus normal neutrophils and monocytes. Keywords = Leukemia Keywords = APL Keywords = AML Keywords = chemical genomics Keywords: repeat sample

ORGANISM(S): Homo sapiens

PROVIDER: GSE976 | GEO | 2004/01/30

SECONDARY ACCESSION(S): PRJNA89871

REPOSITORIES: GEO

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2008-10-26 | E-GEOD-12662 | biostudies-arrayexpress

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2008-09-06 | GSE12662 | GEO

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