Transcriptomics

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Lin37 is essential for repression of cell-cycle genes in quiescence


ABSTRACT: The DREAM complex represses cell-cycle gene transcription in quiescence. To investigate the role of the DREAM component Lin37, we created Lin37 knockout cells by CRISPR/Cas9-nickase. pRTS episomal vectors expressing luciferase or Lin37 were introduced in two independent knockout clones (411-27 and 632-2) to create Lin37 knockout and rescue cells. To exit the cell cycle and to enter quiescence, cells were serum starved for 60h. RNA-seq was applied to identify genes de-regulated by loss of Lin37 during quiescence.

ORGANISM(S): Mus musculus

PROVIDER: GSE97716 | GEO | 2017/08/17

SECONDARY ACCESSION(S): PRJNA382716

REPOSITORIES: GEO

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