Genomics

Dataset Information

0

Tissue-specific CTCF/Cohesin-mediated chromatin architecture delimits enhancer interactions and function in vivo (ChIP-Seq)


ABSTRACT: The genome is organized via CTCF/cohesin binding sites, which partition chromosomes into 1-5Mb topologically associated domains (TADs), and further into smaller contact sub-domains within TADs (sub-TADs; 40-1000kb). Here we examined in vivo an ~80kb sub-TAD, containing the mouse α-globin gene cluster, lying within a ~1Mb TAD. We find that the sub-TAD is flanked by predominantly convergent CTCF/cohesin sites which are ubiquitously bound by CTCF but only interact during erythropoiesis, defining a self-interacting erythroid compartment. Whereas the α-globin regulatory elements normally act solely on promoters downstream of the enhancers, removal of a conserved upstream CTCF/cohesin boundary extends the sub-TAD to the adjacent upstream CTCF/cohesin binding site. The α-globin enhancers now interact with the flanking chromatin, upregulating expression of genes within this extended sub-TAD. Rather than acting solely as a barrier to chromatin modification, CTCF/cohesin boundaries in this sub-TAD regulate both directionality and specificity of enhancer interactions with surrounding promoters.

ORGANISM(S): Mus musculus

PROVIDER: GSE97869 | GEO | 2017/06/14

SECONDARY ACCESSION(S): PRJNA383119

REPOSITORIES: GEO

Similar Datasets

2017-06-14 | GSE97870 | GEO
2017-06-14 | GSE97867 | GEO
2017-06-14 | GSE97866 | GEO
2022-06-01 | GSE153205 | GEO
2022-06-01 | GSE153208 | GEO
2022-06-01 | GSE153207 | GEO
2022-06-01 | GSE153206 | GEO
2017-07-12 | GSE76573 | GEO
2018-10-19 | GSE115340 | GEO
2018-05-31 | GSE101921 | GEO