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Rem2 promotes synapse formation by regulating the expression of genes encoding synaptogenic molecules

ABSTRACT: Purpose: Determination of whether knockout of the GTPase Rem2 results in changes in gene expression

ORGANISM(S): Mus musculus

PROVIDER: GSE97891 | GEO | 2017/10/31

SECONDARY ACCESSION(S): PRJNA383148

REPOSITORIES: GEO

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Elimination of the four extracellular matrix molecules tenascin-C, tenascin-R, brevican and neurocan modifies the expression of key molecules of synapse formation and transmission

Project description:The extracellular matrix (ECM) molecules tenascin-C (Tnc), tenascin-R (Tnr), brevican (Bcan) and neurocan (Ncan) are completely knocked out in the quadruple knockout mouse. This removal of these central ECM molecules lead to an altered gene expression in the hippocampus of postnatal day (P) 21 old quadruple knockout mice. Among others, the expression of genes involved in the process of synapse formation associated with synaptic transmission were significantly changed in the quadruple knockout hippocampus compared to the wildtype hippocampus. We found e.g. an upregulated expression of Gamma-aminobutyric acid receptor subunit theta (Gabrq), Glutamate decarboxylase (Gad2) and semaphorin 4c (Sema4c), which are known contributors at synapses. Interestingly, some extracellular molecules e.g. like glypican 3 (Gpc3), calreticulin (Calr) and three different types of collagens (Col1a2, Col4a3 and Col27a1) showed also a modified expression. These data indicate the importance of the four ECM molecules for an intact gene expression in the murine hippocampus.

2019-10-08 | GSE127216 | GEO