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Single cell analysis of atherosclerosis baseline and regression macrophages-surgical model

ABSTRACT: CD45+ CD11b+ F4/80+ macrophages were isolated using the BD FACS Aria IIu SORP from pre-transplant baseline (Apoe-/- fed 16 weeks westernd diet) and grafted arches from WT recipient mice (regression). Cells were then sorted using the C1 Fluidigm to analyze their transcriptomes at a single cell level.

ORGANISM(S): Mus musculus

PROVIDER: GSE97941 | GEO | 2017/05/15

SECONDARY ACCESSION(S): PRJNA383399

REPOSITORIES: GEO

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Dual ontogeny of disease-associated microglia and disease inflammatory macrophages in aging and neurodegeneration

Project description:Mice brain at different age were digested and cell suspension were prepared. Cells were stained using anti-CD45, F4/80, CD11b and Ly6C and sorted using an cell sorter BD ARIA. Macrophages were then prepared in order to perform single cell RNA-Seq

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Human Single Cells - Local lung hypoxia determines epithelial fate decisions during alveolar regeneration

Project description:Single human lung epithelial cell transcriptomes, isolated and processed via Fluidigm C1

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C1 analysis using mixtures of human (HEK) and mouse (3T3) cells

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Gene expression profiling of mouse liver cells

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Project description:5' selective RNA-seq of 76 Single HEK293 cells RNAseq profiling with N6H4 unique molecular identifiers processed on a Fluidigm C1.

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Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of 3 Ewing Sarcoma PDX (PDX-83, 84 and 111) with the Fluidigm C1 technology.

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Project description:We performed Fluidigm C1 single cell sequencing analysis of wild-type and microRNA deficient (Dgcr8 knockout) mouse embryonic stem cells mock treated or transfected with either miR-294 or let-7.

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