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Dynamic Maps of UV Damage Formation and Repair for the Human Genome

ABSTRACT: UV-induced DNA damage is the major carcinogen of skin cancer. We developed HS-Damage-seq to map UV damage with unprecedented sensitivity and resolution in human cells. While the distribution of UV-induced damage are mainly determined by sequence context and essentially uniform throughout the genome, transcription factors can affect damage formation at specific positions. With time-course experiments, we observed changes in damage distribution at particular genomic loci and chromatin states, which reflects the nucleotide excision repair preferences and complements the repair maps by XR-seq. Our results demonstrated that while initial damage is uniformly distributed, they are heterogeneously repaired throughout the genome. The combination of damage and repair maps provides a holistic perspective of UV damage and repair in the human genome.

ORGANISM(S): Homo sapiens

PROVIDER: GSE98025 | GEO | 2017/06/27

SECONDARY ACCESSION(S): PRJNA383707

REPOSITORIES: GEO

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