Genomics

Dataset Information

0

The energetics and physiological impact of cohesin extrusion


ABSTRACT: Cohesin extrusion is thought to play a central role in establishing the architecture of mammalian genomes. However, extrusion has not been visualized in vivo, and thus, its functional impact and energetics are unknown. Using ultra-deep Hi-C, we show that loop domains form by a process that requires cohesin ATPases. Once formed, however, loops and compartments are maintained for hours without energy input. Strikingly, without ATP, we observe the emergence of hundreds of CTCF-independent loops that link regulatory DNA. We also identify architectural "stripes" where a loop anchor interacts with entire domains at high frequency. Stripes often tether super-enhancers to cognate promoters, and in B cells, they facilitate Igh transcription and recombination. Stripe anchors represent major hotspots for topoisomerase-mediated lesions, which promote chromosomal translocations and cancer. In plasmacytomas, stripes can deregulate Igh-translocated oncogenes. We propose that higher organisms have coopted cohesin extrusion to enhance transcription and recombination, with implications for tumor development.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE98119 | GEO | 2018/05/01

REPOSITORIES: GEO

Similar Datasets

2023-05-30 | GSE230251 | GEO
2023-05-30 | GSE230604 | GEO
2023-05-30 | GSE230249 | GEO
2022-03-08 | GSE196300 | GEO
2022-03-07 | GSE155971 | GEO
2022-03-08 | GSE196496 | GEO
2022-03-14 | PXD032185 | Pride
2017-08-25 | GSE100569 | GEO
2023-01-15 | GSE210289 | GEO
2022-08-15 | GSE199059 | GEO