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Function of Sox2 and Klf4 during SKM reprogramming

ABSTRACT: Differentiated somatic cells can be reprogrammed into induced pluripotent stem cells by ectopic expression of transcription factors Oct4, Sox2, Klf4, and c-Myc, but the mechanisms are still to be dissected. The stoichiometry of factors influences the efficiency of induced pluripotent stem cells, and previous studies emphasized the requirement of high levels of overexpressed Oct4. In this study, we showed that, with appropriate stoichiometry achieved by polycistronic cassettes, Sox2 and Klf4 were sufficient to initiate and establish pluripotency in differentiated cells efficiently without Oct4 overexpression.

ORGANISM(S): Mus musculus

PROVIDER: GSE98280 | GEO | 2020/01/01

REPOSITORIES: GEO

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Analysis of Klf4 factor stoichiometry effects during iPS cell derivation from mouse embryonic fibroblasts

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2015-03-13 | GSE65468 | GEO

Analysis of gene expression dynamics during iPS cell derivation from mouse embryonic fibroblasts using reprogramming systems with different Klf4 stoichiometry

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2019-01-10 | GSE116309 | GEO

Oct4-Induced Pluripotency in Adult Neural Stem Cells

Project description:The four transcription factors Oct4, Sox2, Klf4, and c-Myc can induce pluripotency in mouse and human fibroblasts. We previously described direct reprogramming of adult mouse neural stem cells (NSCs) by Oct4 and either Klf4 or c-Myc. NSCs endogenously express Sox2, c-Myc, and Klf4 as well as several intermediate reprogramming markers. Here we report that exogenous expression of the germline-specific transcription factor Oct4 is sufficient to generate pluripotent stem cells from adult mouse NSCs. These one-factor induced pluripotent stem (1F iPS) cells are similar to embryonic stem cells in vitro and in vivo. Not only can these cells be efficiently differentiated into NSCs, cardiomyocytes and germ cells in vitro, but they are also capable of teratoma formation and germline transmission in vivo. Our results demonstrate that Oct4 is required and sufficient to directly reprogram NSCs to pluripotency.

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MEF_OSK_O_KS_T20

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2015-09-17 | E-GEOD-61694 | biostudies-arrayexpress

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Determination of the protein interactome of the transcription factor Sox2 in embryonic stem cells engineered for inducible expression of four reprogramming factors

2012-02-20 | GSE34801 | GEO

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2015-08-08 | GSE70816 | GEO

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