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Exposure to Deepwater Horizon Oil and Corexit 9500 at Low Concentrations Induces Transcriptional Changes and Alters Immune Transcriptional Pathways in Sheepshead Minnows

ABSTRACT: To investigate molecular effects in fish following exposure to environmentally relevant concentrations of dispersants and DWH oil, we exposed adult sheepshead minnows (Cyprinodon variegatus) to Corexit 9500™ and two concentrations of high-energy water-accommodated fraction (HEWAF) or chemically enhanced water-accommodated fraction (CEWAF) for 7 and 14 days. Resulting changes in hepatic gene expression were measured using Agilent 8x15K microarrays. We observed significant changes to gene expression in all treatments (relative to controls), with Corexit and CEWAF having a greater effect on expression patterns in the liver than HEWAF treatments. Sub-network enrichment analysis of biological pathways revealed that the greatest number of altered pathways in high dose HEWAF and CEWAF treatments occurred following a 7-day exposure. Pathways related to immunity comprised the majority of pathways affected in each treatment, followed by pathways related to blood and circulation processes.

ORGANISM(S): Cyprinodon variegatus

PROVIDER: GSE98348 | GEO | 2017/04/29

SECONDARY ACCESSION(S): PRJNA384790

REPOSITORIES: GEO

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