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Babesia Infected/Control LongSAGE libraries for Boophilus microplus ovary tissue

ABSTRACT: Babesia infected (and control) ovary tissue was used to construct LongSAGE libraries. The intent was to find differential gene expression in both libraries. Details: The extraction was done using Ambion's ToTALLY RNA kit, and the concatemers where constructed using Invitrogen's I-SAGE Long kit. The concatmer files (multiple fasta sequence files) were processed using in-house perl scripts to extract the 17bp LongSAGE tags. **Note: contact person: Felix D. Guerrero. email: felix.guerrero@ars.usda.gov Keywords: LongSAGE

ORGANISM(S): Rhipicephalus microplus

PROVIDER: GSE9880 | GEO | 2009/03/01

SECONDARY ACCESSION(S): PRJNA103873

REPOSITORIES: GEO

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Microarray analysis of expressed genes from R. microplus larvae, adult ovary, adult female gut associated with B bovis.

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