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Riboflavin depletion regulates cell proliferation and cell cycle progression‑associated genes in HEK293T cells

ABSTRACT: Transcriptome analysis of RNA samples from riboflavin-depleted HEK293T cells. Riboflavin is an essential component of the human diet and its derivative cofactors have an established role in oxidative metabolism. Riboflavin deficiency has been linked with various human diseases. In this study, we have modelled riboflavin deficiency in HEK293T cell line, shown remarkable increase of cell proliferation. The aim of the present study is to develop a cell culture model of riboflavin depletion and analyze its molecular mechanism of acceleration cell proliferation. So, we used Affymetrix Human Transcriptome Array 2.0 to identify genes that were differentially expressed upon riboflavin-depleted HEK293T cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE98927 | GEO | 2017/05/16

SECONDARY ACCESSION(S): PRJNA386885

REPOSITORIES: GEO

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Riboflavin depletion impairs cell proliferation in intestinal cells: Identification of mechanisms and consequences

Project description:Microarray analysis has been applied to the cell proliferation in a human colonic cel line, Caco-2. We have shown previously that a moderate riboflavin depletion around weaning has a profound impact on the structure and function of the small intestine of the rat, which is not reversible following riboflavin repletion. In this study we have modelled riboflavin deficiency in a human cell line, shown irreversible loss of cell viability associated with impaired mitosis and identified candidate effectors of riboflavin depletion in the cell. The aim of the present study is to develop a cell culture model of riboflavin depletion and analyse its behaviour using a using a combination of cell biology approaches including microarray analysis. A human colonic cell line, Caco-2, was grown in culture and treated to riboflavin depletion. Treated and untreated cells were collected at appropriate time points and isolated RNA subjected to microarray analysis.

2009-03-06 | E-GEOD-15132 | biostudies-arrayexpress

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Riboflavin deficiency regulates inflammation-associated genes in rat esophageal intraepithelial neoplasia

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Transcriptomics and Real-Time PCR analysis reveals a cross-modulatory effect between riboflavin and iron and outlines common and specific responses to riboflavin biosynthesis and uptake in Vibrio cholerae

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Transcriptome profiles of a riboflavin-producing B.subtilis RH33 and wild type B.subtilis 168

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2010-05-05 | E-GEOD-12873 | biostudies-arrayexpress

Homo sapiens

Project description:Riboflavin depletion regulates cell proliferation and cell cycle progression‑associated genes in HEK293T cells

| PRJNA386885 | ENA

Riboflavin deficiency regulates inflammation-associated genes in rat esophageal intraepithelial neoplasia

Project description:Riboflavin deficiency regulates inflammation-associated genes in rat esophageal intraepithelial neoplasia

| PRJNA436162 | ENA

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