Genomics

Dataset Information

46

ALG-5 is a miRNA-associated Argonaute required for proper developmental timing in the Caenorhabditis elegans germline


ABSTRACT: Caenorhabditis elegans contains twenty-five Argonautes, of which, only ALG-1 and ALG-2 are known to interact with miRNAs. ALG-5 belongs to the AGO subfamily of Argonautes that includes ALG-1 and ALG-2, but its role in small RNA pathways is unknown. We analyzed by high-throughput sequencing the small RNAs associated with ALG-5, ALG-1, and ALG-2, as well as changes in mRNA expression in alg-5, alg-1, and alg-2 mutants. Overall design: We analyzed by high-throughput sequencing miRNA-Argonaute interactions in C. elegans and their impact on mRNA levels. The data in this series includes mRNA sequencing of loss-of-function mutants, small RNA sequencing of loss-of-function mutants, and sequencing of small RNAs that co-immunoprecipitate with each Argonaute protein.

INSTRUMENT(S): Illumina HiSeq 2500 (Caenorhabditis elegans)

ORGANISM(S): Caenorhabditis Elegans

SUBMITTER: Taiowa Montgomery  

PROVIDER: GSE98935 | GEO | 2017-06-09

SECONDARY ACCESSION(S): PRJNA386876

REPOSITORIES: GEO

Dataset's files

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GSE98935_mRNA_counts.xlsx Xlsx
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Publications

ALG-5 is a miRNA-associated Argonaute required for proper developmental timing in the Caenorhabditis elegans germline.

Brown Kristen C KC   Svendsen Joshua M JM   Tucci Rachel M RM   Montgomery Brooke E BE   Montgomery Taiowa A TA  

Nucleic acids research 20170901 15


Caenorhabditis elegans contains 25 Argonautes, of which, ALG-1 and ALG-2 are known to primarily interact with miRNAs. ALG-5 belongs to the AGO subfamily of Argonautes that includes ALG-1 and ALG-2, but its role in small RNA pathways is unknown. We analyzed by high-throughput sequencing the small RNAs associated with ALG-5, ALG-1 and ALG-2, as well as changes in mRNA expression in alg-5, alg-1 and alg-2 mutants. We show that ALG-5 defines a distinct branch of the miRNA pathway affecting the expre  ...[more]

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