Transcriptomics,Genomics

Dataset Information

159

Gene expression profiling of intact individual planarians during homeostatic tissue turnover after inhibition of myoD or nkx1-1


ABSTRACT: MyoD is a conserved myogenic factor which controls formation of longitundinal muscle fibers in planarians. nkx1-1 is a conserved transcription factor which controls formation of circular muscle fibers in planarians. Overall design: Polyadenylated RNA was isolated from individual animals from planarians following RNAi (10 dsRNA feedings) of a control gene (C.elegans unc-22; 6 replicates) and myoD (6 replicates) or following RNAi (12 dsRNA feedings) of a control gene (C. elegans unc-22; 5 replicates) and nkx1-1 (7 replicates).

INSTRUMENT(S): Illumina HiSeq 2000 (Schmidtea mediterranea)

SUBMITTER: Peter W Reddien 

PROVIDER: GSE99025 | GEO | 2017-11-22

SECONDARY ACCESSION(S): PRJNA387022

REPOSITORIES: GEO

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Publications

Orthogonal muscle fibres have different instructive roles in planarian regeneration.

Scimone M Lucila ML   Cote Lauren E LE   Reddien Peter W PW  

Nature 20171122 7682


The ability to regenerate missing body parts exists throughout the animal kingdom. Positional information is crucial for regeneration, but how it is harboured and used by differentiated tissues is poorly understood. In planarians, positional information has been identified from study of phenotypes caused by RNA interference in which the wrong tissues are regenerated. For example, inhibition of the Wnt signalling pathway leads to regeneration of heads in place of tails. Characterization of these  ...[more]

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