Chilling requirement fulfillment in Vitis riparia overwintering buds
Ontology highlight
ABSTRACT:
ORGANISM(S): Vitis vinifera Vitis riparia
PROVIDER: GSE9903 | GEO | 2009/06/03
SECONDARY ACCESSION(S): PRJNA103895
REPOSITORIES: GEO
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Project description:This study investigated changes in gene expression of controlled environment chilled (4C) grape overwintering buds as they accumulated from 0 to 2000 chilling hours. Keywords: time course, chilling, endodormancy release, axillary bud, grape A loop design with 3 biological replicates (RNA from buds collected at 0, 500, 1000, 1500, and 2000 hr of chilling in 2002, 2004, and 2005).
2010-05-18 | E-GEOD-9903 | biostudies-arrayexpress
Project description:This study compares age matched V. riparia axillary buds at one time point during long photoperiod (paradormancy maintenance) and short photoperiod (endodormancy induced). Keywords: endodormancy, photoperiod, paradormancy, grape, axillary bud
2005-08-05 | GSE3052 | GEO
Project description:The transition of axillary grapevine buds from paradormancy to endodormancy is an important mechanism for winter survival. Maintenance of paradormancy and transition to endodormancy may be synchronized by factors including phytohormones, genes, transcription factors and other molecular regulators. In the present study we used age matched buds from vines subjected to 28 days of short day (SD; 13h) treatment or continued long day (LD; 15h) photoperiod for high throughput transcriptome profiling.
2021-04-28 | GSE95429 | GEO
Project description:In rice (Oryza sativa L.), chilling-induced male sterility increased when plants experienced low water temperature (Tw, 18 M-BM-0C for 14 days) before panicle initiation. The number of mature pollen grains after chilling at the booting stage (12 M-BM-0C for 5 days) was only approximately 45% of total pollen grains in low-Tw plants, whereas it was approximately 71% in normal-Tw plants (Tw not controlled; approximately 23 M-BM-0C under air temperature of 26 M-BM-0C/21 M-BM-0C, day/night). Microarray and quantitative PCR analyses showed that many stress-responsive genes (including OsFKBP65 and genes encoding a large heat shock protein OsHSP90.1, heat shock factors, and many small heat shock proteins) were strongly up-regulated by chilling in normal-Tw spikelets, but were not or rather down-regulated by chilling in low Tw spikelets. OsAPX2 and genes encoding some other antioxidative enzymes were also significantly down-regulated by low Tw in the chilled spikelets. In low-Tw plants, lipid peroxidation products (malondialdehyde equivalents) were significantly increased in the spikelets after chilling, and ascorbate peroxidase activity in the chilled spikelets was significantly lower than that in normal-Tw plants. Our data suggest that an OsFKBP65-related chilling response, which protects proteins from oxidative damage, is indispensable for chilling tolerance but is lost in low-Tw spikelets. Four conditions used: low Tw and chilled, low Tw and not chilled, normal Tw and chilled, normal Tw and not chilled, before panicle initiation and at the booting stage, respectively. Biological replicates: 4 for each treatment.
2014-12-22 | E-GEOD-59221 | biostudies-arrayexpress
Project description:Bud endodormancy induction response of two genotypes (Seyval, a hybrid white wine grape and Vitis riparia, PI588259, a native North American grape species) was compared under long (15 h) and short (13 h) photoperiods. Proteins were extracted from both genotypes for all time points and experimental conditions. The proteins were separaed by 2D-PAGE, trypsin digested, and the peptides identified with a MALDI-TOF-TOF mass spectrometer. A master gel was made and mapped with all proteins from both genotypes. The proteins were identified by matching the peptide sequences against the 8X Vitis vinifera grape genome in NCBI. This study was funded by NSF grant DBI064755 and is the result of a collaboration between Dr. Anne Fennell at South Dakota State University and Dr. Grant R. Cramer at the University of Nevada, Reno.
2010-08-09 | PRD000169 | Pride
Project description:In rice (Oryza sativa L.), chilling-induced male sterility increased when plants experienced low water temperature (Tw, 18 °C for 14 days) before panicle initiation. The number of mature pollen grains after chilling at the booting stage (12 °C for 5 days) was only approximately 45% of total pollen grains in low-Tw plants, whereas it was approximately 71% in normal-Tw plants (Tw not controlled; approximately 23 °C under air temperature of 26 °C/21 °C, day/night). Microarray and quantitative PCR analyses showed that many stress-responsive genes (including OsFKBP65 and genes encoding a large heat shock protein OsHSP90.1, heat-stress transcription factors, and many small heat shock proteins) were strongly up-regulated by chilling in normal-Tw spikelets, but were not or rather down-regulated by chilling in low Tw spikelets. OsAPX2 and genes encoding some other antioxidative enzymes were also significantly down-regulated by low Tw in the chilled spikelets. In low-Tw plants, lipid peroxidation products (malondialdehyde equivalents) were significantly increased in the spikelets after chilling, and ascorbate peroxidase activity in the chilled spikelets was significantly lower than that in normal-Tw plants. Our data suggest that an OsFKBP65-related chilling response, which protects proteins from oxidative damage, is indispensable for chilling tolerance but is lost in low-Tw spikelets.
2014-12-22 | GSE59221 | GEO
Project description:In deciduous fruit trees, entrance into dormancy occurs in later summer/fall, concomitantly with the shortening of day length and decrease in temperature. Generally speaking, dormancy can be divided into endodormancy, ecodormancy and paradormancy. In Prunus species flower buds, entrance into the dormant stage occurs when the apical meristem is partially differentiated; during dormancy, flower verticils continue their growth and differentiation. In this work we focused our attention on flower bud development during winter in peach. In order to understand how bud development progress is regulated during winter we integrated cytological epigenetic and chromatin genome wide data with transcriptional outputs to obtained a complete picture of the main regulatory pathways involved in endodormancy.
2022-04-06 | GSE190586 | GEO
Project description:In deciduous fruit trees, entrance into dormancy occurs in later summer/fall, concomitantly with the shortening of day length and decrease in temperature. Generally speaking, dormancy can be divided into endodormancy, ecodormancy and paradormancy. In Prunus species flower buds, entrance into the dormant stage occurs when the apical meristem is partially differentiated; during dormancy, flower verticils continue their growth and differentiation. In this work we focused our attention on flower bud development during winter in peach. In order to understand how bud development progress is regulated during winter we integrated cytological epigenetic and chromatin genome wide data with transcriptional outputs to obtained a complete picture of the main regulatory pathways involved in endodormancy.
2022-04-06 | GSE189882 | GEO
Project description:Bud dormancy – the repeated phase of rest that punctuates periods of growth in the life cycles of many perennial species. In temperate fruit trees, fulfillment of chilling requirement and subsequent heat requirement enable dormant buds to have uniform blooming in field. However, effects of environmental factors such as chilling underlying dormancy release and bud break has not been fully understood. Histone modification is an important epigenetic regulation system which plays an important role in gene expression in various developmental and adaptive processes. Taking advantage of next-generation sequencing, we generated epigenome and transcriptome profiling at different stages before chilling, after chilling and just before bud break in apple (Malus x domestica). We found H3K27me3 may play dominant role during chilling and the genes involved in lignin and lipid metabolic process showed histone modifications. Interestingly, the higher ratio of genes in chilling-associated network exhibited histone modifications, suggesting crucial epigenetic roles in regulating gene expressions in response to chilling during dormancy. Furthermore, H3K4me3 may play more important role during bud break and the genes related to cell wall modification/organization were strongly modified. Taken together, this study provides important insights into the chromatin-based gene regulation underlying chilling-mediated dormancy release and bud break in apple.
2024-03-31 | GSE128041 | GEO
Project description:Bud dormancy – the repeated phase of rest that punctuates periods of growth in the life cycles of many perennial species. In temperate fruit trees, fulfillment of chilling requirement and subsequent heat requirement enable dormant buds to have uniform blooming in field. However, effects of environmental factors such as chilling underlying dormancy release and bud break has not been fully understood. Histone modification is an important epigenetic regulation system which plays an important role in gene expression in various developmental and adaptive processes. Taking advantage of next-generation sequencing, we generated epigenome and transcriptome profiling at different stages before chilling, after chilling and just before bud break in apple (Malus x domestica). We found H3K27me3 may play dominant role during chilling and the genes involved in lignin and lipid metabolic process showed histone modifications. Interestingly, the higher ratio of genes in chilling-associated network exhibited histone modifications, suggesting crucial epigenetic roles in regulating gene expressions in response to chilling during dormancy. Furthermore, H3K4me3 may play more important role during bud break and the genes related to cell wall modification/organization were strongly modified. Taken together, this study provides important insights into the chromatin-based gene regulation underlying chilling-mediated dormancy release and bud break in apple.
2024-03-31 | GSE171131 | GEO