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RNA-seq for understanding effects of vitamin B12 removal on astrocyte culture

ABSTRACT: Astrocytes express a vitamin B12 uptake receptor, CD320/TCblR, that is regulated by S1P1 signaling. In search of genes controlled under B12 deficiency in astrocytes, we employed RNA-seq using RNA of astrocytes cultured in normal or B12 deficient media.

ORGANISM(S): Mus musculus

PROVIDER: GSE99113 | GEO | 2022/05/17

REPOSITORIES: GEO

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RNA-seq for understanding effects of vitamin B12 removal on astrocyte culture and understanding astrocyte S1P1 signaling

Project description:RNA-seq for understanding effects of vitamin B12 removal on astrocyte culture and understanding astrocyte S1P1 signaling

| PRJNA387305 | ENA

RNA-seq for understanding astrocyte S1P1 signaling

Project description:Astrocytic S1P1 has been previously shown to be essential for FTY720 (an orally delivered drug for MS) efficacy (CNS), in addition to its well-known immunological mechanisms of action. FTY720-P (an analogue of S1P) is a functional antagonist to S1P1. TetTag c-Fos reporter mice displayed temporal and spatial GFP-labeling of astrocytic nuclei under experimental autoimmune encephalomyelitis (EAE) pathology. We generated astrocyte conditional S1P1-KO mice in c-Fos background (S1P1-AsCKOfos). In search of essential factors controlled by S1P-S1P1 signaling in astrocytes, we employed nuclear RNA-seq using RNA of DAPI+NeuN-GFP+ nuclei isolated from spinal cords (SCs) of EAE-induced WTfos, S1P1-AsCKOfos, and FTY720-treated WTfos mice.

2022-05-17 | GSE99114 | GEO

Transthyretin orchestrates vitamin B12-induced stress resilience

Project description:Background: Chronic stress significantly contributes to mood- and anxiety disorders. Previous data suggest a correlative connection between vitamin B12 supplementation, depression, and stress resilience. However, the underlying mechanisms are still poorly understood. Methods: Using the chronic variable stress mouse model coupled with RNA-sequencing, we determined vitamin B12-induced transcriptional changes related to stress resilience. By viral-mediated gene transfer and in vivo epigenome editing, we reveal a functional pathway linking vitamin B12, DNA methylation, and depressive-like symptoms. Results: We identified Transthyretin (Ttr) as a sex-specific key target of vitamin B12 action in chronic stress. Accordingly, TTR expression was increased postmortem in the prefrontal cortex of male, but not female, depressed patients. Virally altered Ttr in the prefrontal cortex functionally contributed to stress- and depression-related behaviors, changes in dendritic spine morphology, and gene expression. In stressed mice, vitamin B12 reduced DNAme in the Ttr promoter region. Importantly, using in vivo epigenome editing to alter DNAme in the brains of living mice for the first time, we establish a direct causal link between DNAme on Ttr and stress-associated behaviors. Discussion: In summary, using state-of-the-art techniques, this study uncovers a mechanistic link between cobalamin supplementation, Ttr, and markers of chronic stress and depression, encouraging further studies into dietary interventions for mood disorders.

2024-05-13 | GSE267047 | GEO

New Roles for Vitamin B12 Regulation

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RNA-seq for understanding effects of vitamin B12 removal on astrocyte culture and understanding astrocyte S1P1 signaling

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-05-17 | GSE99115 | GEO

Mutations of the selected strain growing in low levels of Vitamin B12 in Mycobacterium marinum

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2012-06-17 | E-GEOD-34836 | biostudies-arrayexpress

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2010-05-17 | E-GEOD-13391 | biostudies-arrayexpress

Effect of amino acid availability on vitamin B12 production in Lactobacillus reuteri

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