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H3K36me3 protects the mouse epigenome from single nucleotide variations [mRNA-Seq]

ABSTRACT: The goals of this project are to study the transcriptome profiling (RNA-seq) of mouse embryonic stem cells by histone mutants incorporation (H3.1K3M, H3.1K36M vs H3.1wt) or shRNA-mediated knockdown of methyl-transferase Suv39h1/h2 (shSuv39h1, shSuv39h2 vs shNC)

ORGANISM(S): Mus musculus

PROVIDER: GSE99155 | GEO | 2019/08/01

REPOSITORIES: GEO

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H3K36me3 protects the mouse epigenome from single nucleotide variations

Project description:We adopt histone H3 mutants competitive incorporation system to screen the effects of them on genetic fidelity. We find incorporation of H3K36M, an oncogenic histone mutant, produce a cancer or ageing-like genetic landscape by punctuating the H3K36me3/H3K9me3 dependent DNA repair mechanism. This work firstly get a direct relationship between epigenetic inheritance and genomic stability

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Project description:We analyze high-throughput profiling of histone H3K36me3 in mouse embryonic stem cells from chromatin immunoprecipitated DNA captured by H3K36me3-specific antibody. We find that H3K36me3 distribution is like expected and enriched at transcribing gene bodies especially with enrichment at 3’-terminal of gene body, which is identical with findings observed in other mammalian cells.

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H3K36me3 protects the mouse epigenome from single nucleotide variations

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